摘要
采用多孔聚氨酯泡沫对Penicillium sp.ZD-Z1菌进行了固定化,在鼓泡床生化反应器中同时产酶降解壳聚糖,在30℃下pH5.0通气量为0.1m3/h条件下,连续进行18批同时产酶降解试验,结果发现壳聚糖酶活力和壳聚糖降解效率能保持稳定,每批产生的壳聚糖酶活力可达0.128U/mL(发酵液)以上,壳聚糖的粘均分子量可以降解到4ku以下,通过控制培养时间就可以得到重均分子量为120172、28425、16448、5599,峰尖分子量17579、111583、111235、3351的壳低聚糖。利用酶法降解所得的低分子量壳聚糖在经初步的分离纯化后,经红外光谱分析后,壳聚糖在酶解前后主要峰位置没有变化---壳聚糖的化学结构并未发生改变,这表明固定化Penicillium sp ZD-Z1菌产酶降解壳聚糖反应易于控制,不改变壳聚糖的化学结构,是一种有效的制备高品质壳低聚糖的方法。
The cells of Penicillium sp. ZD-Zlwere immobilized in the matrix of porous polyurethane foam and packed in a bubbling bioreaetor for simultaneous production of chitosanase and degradation of chitosan in situ. After the repeated-batch process with 18 times under the conditions of pHS. 0, 30℃ and 0. 1m^3/h ventilate rate, the bioreaetor system run stably and effectively without notable change in the activity and productivity. The activity of chitosanase for each batch was above 0. 128U/mL, and the average viscosity molecular weight could reach 12017 2,28425,16448 and 5599 only by control the time of degradation. Through infrared spectroscopic analysis, the chemical configuration of low molecular weight ehitosan have little breakage, the primary peak amplitude was the same with high molecular weigh chitosan,which indicated that chitosan degradation by immobileized Penicillium sp. ZD-Z1 was a good method of high quality chitosan production.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2008年第8期28-31,36,共5页
Food and Fermentation Industries
