人参四逆汤质量控制方法的研究——乌头类生物碱的鉴别、含量测定及乌头碱的限量检查

STUDIES ON THE METHOD OF QUALITY CONTROL OF RENSHEN SINITANG——IDENTIFICATION TEST、QUANTITATIVE DETERMINATION OF ACONITE ALKALOIDS AND LIMIT TEST OF ACONITINE

本文以人参四逆汤中附片的有效成分为指标。用薄层色谱法作乌头类生物碱鉴别及乌头碱的限量检查。用离子对萃取—分光光度法测定乌头类生物碱含量,乌头碱的回收率为98.33%±4.72%～103.6±3.09%,加样回收率为95.09±2.09%。方法准确、重现性好。

Renshen Sinitang is a formula of Chinese traditional medici- nend meteria medica. A method of the quality control of preperation was repo- rted. Both identification test of aconite alkaloids and limit test of aconitine we- re applied with TLC. Quantitative det- ermination of aconite alkaloids was ap- plied with ion pair extraction spectrop- hotometric method. The recovery of the aconitine in prepertion was 98.33±4.72% ～103.6±3.09% (n=20). The internal quality of compound preperations can be controlled with this method.