摘要
目的:探讨SH2-Bβ在哮喘发病中的作用。方法:用卵蛋白致敏致喘的方法制备BALB/c小鼠哮喘模型。应用免疫组织化学方法和免疫印记法(Western blot)观察SH2-Bβ在哮喘小鼠下呼吸道及内脏感觉传入部位的表达及应用SH2-Bβ抗体后SH2-Bβ水平的变化,Metamoph图象分析系统对结果进行分析。结果:免疫组织化学结果显示哮喘小鼠肺、C7-T5节段脊神经节及对应的脊髓后角SH2-Bβ免疫阳性产物平均光密度值分别为0.806±0.023、0.766±0.018、0.547±0.014,明显高于对照组(0.243±0.018、0.131±0.011、0.215±0.029)(P<0.01)。而应用SH2-Bβ抗体组相应部位的平均光密度值分别为(0.252±0.015、0.158±0.012、0.251±0.024)明显低于哮喘组(P<0.01)。Western blot结果显示:哮喘组肺及C7-T5节段脊髓后角SH2-Bβ与β-actin MOD值的比(2.512±0.021,2.615±0.022)明显高于对照组(0.675±0.019,0.712±0.018)(P<0.01),而应用SH2-Bβ抗体组相应部位的比值(0.969±0.023,1.023±0.022)明显低于哮喘组(P<0.01)。结论:肺内、C7-T5脊神经节及对应脊髓后角神经元的SH2-Bβ参与哮喘的发病过程,anti-SH2-Bβ可下调SH2-Bβ的表达,减轻肺部炎症。
Objective: To study the effect of SH2-Bβ on asthma. Methods: Asthma was induced in BALB/c mice by exposure to chicken egg ovalbumin (OVA). Immunohistochemistry and Western blot were used to investigate the changes of SH2-Bβ immunoreactivity and the regulatory effect of anti- SH2-Bβ in the lower respiratory tract and viscerosensory afferent sites of the asthmatic mice. And results were analyzed by the Metamoph analysis system. Results: Immunohistochemistry demonstrated that the mean optic density (MOD) of SH2-Bβ positive substance in the lungs, C7-T5 spinal ganglia and the corresponding posterior horn of the spinal cord (0. 806 ± 0. 023, 0. 766 ± 0. 018, 0. 547 ± 0. 014) of the asthmatic mice was much higher than those ( 0. 243 ± 0. 018, 0. 131 ± 0. 011, 0. 215 ±0. 029) in normal control group respectively ( P 〈 0. 01 ), but MOD values (0. 252 ± 0. 015, 0. 158 ± 0. 012, 0. 251±0. 024 ) respectively in anti- SH2-Bβ group were much lower than those in the asthmatic mice (P 〈0. 01 ). Western Blot demonstrated that the mean ratio of SH2-Bβ MOD to β-actin MOD in lung and spinal cord was (2. 512 ±0. 021, 2.615 ±0.022) in asthmatic group, much higher than (0. 675 ± 0. 019, 0. 712 ± 0. 018 ) in normal control group ( P 〈 0. 01 ) ; and those (0. 969 ± 0. 023, 1. 023 ±0. 022) in anti- SH2-Bβ group was much lower than those of asthmatic mice (P〈0. 01 ). Conclusion: The present results indicate that the SH2-Bβ in the lungs, primary afferent neurons ( C7-T5 spinal ganglia and the corresponding posterior horn of the spinal cord) might be involved in the pathogenesis of asthma. Anti- SH2-Bβ down-regulates the expression of the SH2-Bβ in these portion and reduce the inflammation in the lungs of asthmatic mice.
出处
《沈阳医学院学报》
2008年第3期133-136,共4页
Journal of Shenyang Medical College
基金
辽宁省教育厅基金资助项目(No.20060890)
沈阳医学院优秀人才基金资助项目(No.20073022)