摘要
为了建立仙客来稳定的快繁增殖体系,以仙客来种苗为试材,接种于含不同浓度激素组合的1/2MS培养基上,经过初培养和多次继代培养,诱导产生胚性愈伤,结果表明:球茎是较好的诱导胚性愈伤的材料,1/2MS+6-BA0.2mg/L+2,4-D2mg/L+蔗糖30g/L+琼脂6.5g/L+肌醇100mg/L+酪蛋白100mg/L是较好的诱导配方,将胚性愈伤转接到不含激素的1/2MS基本培养基上,诱导产生大量球形胚和鱼雷胚,2-3周后正常成熟的体胚萌发,表明此研究可以为仙客来的快速繁殖提供技术参考.
To develop a fast and easy reproducible propagation system for Cyclamen persicum Mill. , the seedings are inoculated on 1/2MS medium supplemented with different hormones. Embryogenic callus is induced from tubers of seedlings after culture and subcultures. The results show that the tubers are good explants and 1/2 MS ＋ 6-BA 0.2 mg/L ＋ 2,4-D 2 mg/L ＋ sucrose 30 g/L ＋ agar 6.5 g/L ＋ inositolum 100 mg/L ＋ casein 100 mg/L is the optimal culture medium for induction of embryogenic callus. The regeneration of somatic embryos in high frequencies is observed after plating the embryogenic callus on hormone-free medium. The normal and mature somatic embryos germinate after 2-3 weeks.
出处
《烟台大学学报（自然科学与工程版）》
CAS
2008年第4期281-285,共5页
Journal of Yantai University(Natural Science and Engineering Edition)
基金
山东省科技厅技攻关项目(2004GG4202010)
关键词
仙客来
球茎
胚性愈伤
Cyclamen persicum Mill.
tubers
embryogenic callus
