用~1H-NMR谱对大鼠糖尿病性白内障和正常晶状体山梨醇含量变化的研究

Detection of Sorbitol Content in Crystalline Lens of Normal Rats and Rats with Diabetic Cataract by ~1H-NMR

作者用傅里叶脉冲核磁共振谱仪对大鼠糖尿病性白内障形成过程和大鼠正常晶状体进行了测定,并对其所得谱线加以比较。发现:在δ=3.20ppm处,糖尿病大鼠的峰值有明显变化;在δ=3.73ppm处,随病变发展,其峰值不断增大。表明随山梨醇浓度升高,晶状体内含水量不断增加。此结果与其他学者用生化方法所得结论一致,但它精度高、重复性好。

The pulse Fourier NMR was employedto measure the artificial diabetic cataractlens at various stages of its formation, andthe lenses of the normal rats.Data obtain-ed by using this method show that allthe peaks that of water concentrate in therange of δ<4 ppm. The Peak value atδ= 3.20 ppm is on a marked increase dur-ing the formation of cataract which iscaused by the phosphate metabolites, suchas GPC, ATP, …etc, in cataract lens. Withthe dpvelopment of the disease, the peakwidth at δ= 3.73 ppm becomes greater andgreater, which shows that the activity ofsorbitol dehydrogenase has decreased. Thisleads to a high concentration of the sor-bitol in the cataract lens. Consequently,the osmosis pressure in the cataract lensis increased, and excessive water mightdip into the crystalline lens to keep thebalance of the osmosis pressure. And thismight result in the hydration of the fibercell of the crystalline lens, which mightcause a swelling or blisters. These results are in favour of theprolongation of the relaxation time ofcataractous lens reported in our otherpapers, and also support those gained bybiochemical studies issued in the medicalliterature.