三羟异黄酮对大鼠心肌缺血-再灌注损伤的保护作用

Protective Effectiveness of Genistein on Myocardial Ischemia-reperfusion Injury in Rats

目的观察三羟异黄酮(GST)对大鼠心肌缺血-再灌注损伤(IRI)的影响,并探讨其机制。方法应用大鼠心肌缺血-再灌注模型,观察GST对再灌注心律失常的影响,并测定其血清乳酸脱氢酶(LDH)活性、磷酸肌酸激酶(CK)活性、丙二醛(MDA)含量和超氧化物岐化酶(SOD)活性。结果GST能显著降低再灌注心律失常的发生率,降低室颤发生率,缩短心律失常持续时间,降低血清LDH、CK、MDA的含量,提高血清SOD的活性。结论GST能抑制IRI过程中心肌电活动紊乱,对心肌有明显的保护作用,其机制之一可能与其提高抗氧化酶活性,减少脂质过氧化反应,保护细胞的结构完整性有关。

Objective To study the effects and mechanisms of genistein （GST） on myocardial ischemia-reperfusion injury in rats. Methods The left coronary anterior descending branch was ligated and then reperfused. The incidence, duration of arrhythmia and the contents of serum lactate dehydrogenase （ LDH ）, creatine kinase （ CK ）, malondialhyde （ MDA ）, and superoxide dimutase （SOD） were measured. Results Compared with the control, GST reduced remarkably the incidence of arrhythmia, ventricular fibrillation, shortened the duration of arrhythmia, decreased the content of LDH, CK , MDA and increased the content of SOD. Conclusion GST has a obvious protective-effect against myocardial ischemia-reperfusion injury, and which may be associated with free oxygen radicals scavenging and ultrastructure maintaining activity.