磷脂酶A_2在大鼠急性胰腺炎胰实质坏死中作用的研究

Effect of Phospholipase A_2 on Pancreatic Parenchymal Necrosis ub Acute Pancreatitis in Rats

作者分别将生理盐水、磷脂酶A_2(PLA_2)、卵磷酯(PC)、PLA_2与PC混合液注入大鼠胰胆管,发现PLA_2与PC混合液注入后12小时即已引起急性坏死性胰腺炎,该组胰腺溶血卵磷脂(LPC)占胆碱类磷脂(LPC+PC)的百分比及坏死面积百分比,均明显高于其余三组(分别高达4倍及15倍)。LPC/(PC+LPC)值与坏死程度之间呈明显正相关(r=0.9001,P<0.01)。其余三种处理均未引起明显的胰组织坏死。结果提示,胆汁中PC的存在是形成胆汁性胰腺炎的重要因素,PLA_2作用于PC产生的LPC可能是急性胰腺炎胰实质坏死的起始因素。

The dffect and mechanism of phospho-lipase A_2 (PLA_2) on pancreatic paren-chymal necrosis in acute pancreatitis(AP)in rats were studied. Normal saline(NS). PLA_2,phosphatidylcholine(PC), andPLA_2 mixed PC were respectively injectedinto the biliopancreatic duct of the rat. Themixture of PLA_2 and PC was found toresult in necrotizing AP in the rat in 12hours after injection of the reagent. Thelysophosphatidylcholine (LPC) percentagein the amount of PC and LPC and theextent of pancreatic necrosis in this groupincreased much more significantly thanthose in other three groups(over about4 and 15 times).There is evidently posi-tive correlation(r =0.9001, P<0. 01)betweenLPC percentage and necrosis extent. Theintraductal injection of PLA_2 as well asNS or PC.failed to induce severe morpho-logical changes in the pancreases. Theseresults suggest that PC in bile is neces-sary in the pathogenesis of biliary pan-creatitis and that LPC originated frombile PC by PLA, may be the initiator inpancreatic parenchymal necrosis in AP.