摘要
通过将人组织激肽释放酶(human tissue kallikrein,KLK1)cDNA克隆入自行构建的鸡输卵管特异表达载体pOV中,获得重组载体pOVK经脂质体包裹后,经翅静脉等途径注射产蛋鸡,在收集的鸡蛋蛋清中检测到KLK1活性,表达量高达45U/mL蛋清,有效表达持续7d左右。用含2～4UKLK1的蛋清饲喂高血压大鼠,可使其血压降低70mmHg,5d后回升至饲喂前水平。试验结果表明,在鸡蛋清中表达的重组KLK1口服具有降血压作用,可用于研制降血压保健蛋。
To develop recombinant human tissue kallikrein（KLK1）,KLK1 cDNA was subcloned into chicken oviduct- specific expression vector pOV and the recombinant vector pOVK mixed with polyethyleneimine was injected into laying hens via wing vein. The enzymatic activity was detected in egg white from the vector-injected hens with an maximal expression level of 45 U/mL,which lasted for 7 days. Oral administration of hKLK-contained egg white had significant hypotensive effect on spontaneous hypertension rats,which lasted for 5 days. These results indicated that a possible way was available for developing antihypertension egg by producing egg white containing the recombinant hKLK1 with chicken oviduct bioreactor.
出处
《中国家禽》
北大核心
2008年第18期27-29,共3页
China Poultry
关键词
人组织激肽释放酶
鸡输卵管表达
降血压鸡蛋
human tissue kallikrein
oviduct-specific expression
antihypertension egg
