肺癌组织中CTGF和WISP-1基因表达的研究

Study on expression of CTGF and WISP-1 genes in human lung cancers

目的研究肺癌中CTGF和WISP-1基因表达与临床病理因素的关系。方法采用RT-PCR和免疫组化染色法对60名肺癌患者的癌组织和癌旁正常肺组织中CTGF和WISP-1表达水平进行测定。结果65%(39/60)的肺癌组织CTGF基因表达水平低于癌旁正常肺组织(t=-1.59,P=0.016);83%(50/60)的肺癌组织WISP-1基因表达水平高于癌旁正常肺组织(t=4.15,P=0.000),此结果得到免疫组化的证实。Pearson相关分析:WISP-1和CTGF基因的表达呈显著负相关(r=-0.299;P=0.020)。多元线性回归分析:肿瘤位置和性别对癌组织CTGF基因表达有重要影响;病理类型、年龄和家族史对WISP-1基因表达有影响。结论CTGF和WISP-1基因中单个基因或两个基因相互作用对肺癌的进展均起重要作用及其与临床病理因素的关系。

Objective To investigate CTGF and WISP-1 genes expression levels and theirs relations to clinical and pathological features in human lung carcinomas.Methods The CTGF and WISP-1 mRNA expression levels in samples from sixty primary lung cancers and their matched normal lung tissues were quantified by quantitative real-time PCR assay and immunohistochemistry staining.Results Down-regulations of CTGF gene were quantified found in 65%（39/60） primary lung cancers in comparison the paired normal lung tissues（t=-1.59,P=0.016）.The up-regualtions of WISP-1 gene were observed in 83%（50/60） lung cancers in comparison their normal counterparts（t=4.15,P=0.000）.These results were further conformed by immunohistochemistry staining.Pearson＇s correlation analysis showed that WISP-1 was negatively associated with CTGF（r=-0.299,P=0.020）.Multiple linear regression analysis revealed that the position of the tumor and sex were key factors for CTGF expression,and tumor type,age,family history were valuable predictors for WISP-1 expression.Conclusion These results suggested that CTGF and WISP-1 could play an important role in the progression of primary lung cancers by either individual gene itself or two-gene co-interactions of these genes and theirs relations to clinical and pathological features.