血浆TPS在肺部肿瘤诊断中的研究

Investigation of plasma TPS in lung tumor's diagnosis

目的探讨血浆(肝素钠抗凝)作为标本检测TPS水平的可行性及血浆TPS水平对肺部肿瘤患者的诊断意义。方法采用ELISA法测定20例健康人血浆(肝素钠抗凝)和血清TPS水平,测定63例肺部肿瘤患者血浆TPS水平,采用ROC曲线确定血浆TPS的切割值,以其血清CEA值作为对照。结果健康人血浆和血清TPS水平差异无显著意义(P>0.05);肺癌患者血浆TPS水平明显高于肺部良性肿瘤患者(P<0.001);血浆TPS诊断肺癌灵敏度高于血清CEA(P<0.005),特异度比较差异无统计意义(P>0.05);同健康人相比较,肺部良性肿瘤组血浆TPS增高,差别具显著意义(P<0.01)。结论血浆(肝素钠抗凝)作为标本检测TPS水平有可行性;血浆TPS水平对于肺部肿瘤的诊断有意义,优于血清CEA。

Objective To explore the feasibility of detecting the TPS level using plasma sample, and the significance of the plasma TPS level in the diagnosis of lung tumor.Methods 20 healthy human plasma （heparin prevents clotting） and serum levels of the TPS were measured by enzyme linked immunosorbent assay （ELISA）; 63 cases suffering lung tumor were included, whose plasma levels of TPS were measured. The cut-off value of preoperative plasma TPS was determined by using the ROC curve, serum CEA levels ascontrol. Results There was no significant difference between plasma and serum levels of the TPS in healthy people （P〉0.05）;The plasma TPS levels in lung cancer patients were obviously higher than that in lung benign tumor patients （P〈0.001）; There sensitivity of diagnosising lung tumor using plasma PTS was obviously higher than using serum CEA（P〈0.005）, the contrast of specificity have no significant diference（P〉0.05） ;Compared with the healthy people, the plasma TPS levels in lung benign tumor patients were significantly higher （P〈0.01）. Conclusion It is feasible to detect the levels of the TPS using plasma（heparin prevents clotting） as samples; Plasma TPS levels of preoperative lung tumor patients are meaningful for the diagnosis, better than using serum CEA.