摘要
[目的]建立特异、敏感、快速的脑膜炎奈瑟菌检测方法。[方法]分别以Nm保守ctrA基因、荚膜表达基因orf-2和siaD基因设计引物,根据PCR扩增的特异性产物对Nm进行鉴定与分群。[结果]PCR扩增NmctrA基因得到111bp的特异性产物,非Nm菌株结果为阴性;再以PCR扩增orf-2和siaD基因,分别得到大小400bp(A群)、450bp(B群)、250bp(C群)的特异性片断。对60株Nm试验菌进行PCR鉴定,其结果与血清学分型结果一致。[结论]所建立方法可用于Nm的快速诊断。
[ Objective] To establish a specific, sensitive and rapid method for detection and typing of N.meningitidis. [Methods] The PCR-based methods evaluated in this study included an initial test using the ctrA gene to diagnose N.meningitidis, and test for serogroup identification using the orf-2 gene for serogroup A and the siaD gene for serogroup B, C. [Resuits] The sizes of PCR amplicons were respectively 11 1bp, 400bp, 450bp and 250bp while the genes tested in PCR assay. 60 isolates of N. meningitidis were determined, and the PCR results were equivalent to serology grouping. [ Conclusion] The method we established can be used in clinical diagnosis and epidemiological investigation of meningitis.
出处
《现代预防医学》
CAS
北大核心
2008年第19期3774-3776,共3页
Modern Preventive Medicine
关键词
脑膜炎
PCR
脑膜炎奈瑟菌
诊断
Meningitis
Polymerase chain reaction
Neisseria meningitidis
Diagnosis
