貉被毛中微量元素的ICP-AES分析

Analysis of Trace Elements in Fur of Nyctereutes by ICP-AES

采用低温干灰化法制备样品，用电感耦合等离子体原子发射光谱（ICP-AES）法同时分析毛皮动物貉的被毛组织中多种微量元素。Ba，Be，Cd，Si，Y，zn的测定下限为1.0-9.0ng／g级，B，Fe，La，Mg，Mo，Ni，Ti，Co，Cr，Cu，Sr，V，Zr的测定下限为0.01-0.09μg／g级，In，Th，P，Pb的测定下限为0.13-0.23μg／g级，Nb为1.08μg／g级。经国家人发标准物质（GBW07601）验证，测得该方法相对误差为0.83％-9.59％，相对标准偏差为0.81％-5.20％，各项检测指标均能满足生物样品检测要求。分别测得健康种貉、自咬症轻重病貉被毛中的22种微量元素，健康貉的B，Co，Cr，Ni微量元素含量明显高于病貉，而Al，Ba，Be，Cd，Mo，Pb，Sr，Ti，Zr元素含量较低。

The sample was prepared by low temperature dry ashing. Trace elements of fur tissue on fur-bearing animal （sludraccoon） were simultaneously analysed by inductively coupled plasma-atomic emission spectrometry （ICP-AES）. The lowest quantitative determinable concentrations are between 1.0-9.0μg/g. grade for trace elements Ba,Be,Cd,Si,Y and Zn, and are between 0.01-0.09 μg/g grade for microelement B, Fe, La,Mg,Mo,Ni,Ti,Co,Cr,Cu,Sr,V and Zr, and are between 0.13 -0.23μg/g grade for In,Th,P and Pb. The lowest quantitative determinable concentration of Nb is 1.08μg/g. This method was verified by national hairs standards GBW07601. The relative error is between 0.83% -9.59%. The relative standard deviation is between 0.81%-5.20%. The 22 elements were analysed for the fur of health and illness sludraeeoon. The contents of elements B, Co, Cr and Ni of the health sludraccoon are apparently higher than the corresponding ones of illness sludraccoon. The content of elements Al, Ba, Be, Cd, Mo, Pb, Sr, Ti and Zr of the health sludraccoon were lower than the corresponding ones of illness sludraeeoon. The determination data satisfy the standards of biological sample analysis.