大鼠创伤组织中CGRP,VEGF表达的变化及意义

Changes and forensic pathological significance of the expressions of CGRP and VEGF in rat′s injury

目的:探讨创伤组织中降钙素基因相关肽(CGRP),血管内皮生长因子(VEGF)表达与损伤时间的相关关系。方法:应用免疫组织化学方法、图像分析技术动态检测大鼠创伤组织中CGRP,VEGF的表达。结果:生前创伤组(1,2,4,8,12,24 h)大鼠皮肤组织内CGRP在神经纤维中表达明显高于正常对照组(P<0.05),死亡组大鼠皮肤组织中CGRP表达与正常对照组比较无明显变化(P>0.05)。生前损伤组中CGRP表达在损伤后1 h明显增多,4 h达到高峰,8 h开始下降,以后逐渐恢复到正常。VEGF在损伤后8 h皮肤组织中开始表达,72 h表达最强(P<0.01),96 h开始下降。结论:损伤组织中CGRP表达明显早于VEGF,而且具有时间的规律性,可作为早期创口损伤时间推断中的重要指标。

Objective ： To investigate the relationship of the time of injury and the expressions of calcitonin gene-related peptide（CGRP） and vascular endothelial growth factor （VEGF） in rat＇s skin after injury. Methods： The expressions of CGRP and VEGF in the wound tissue were detected by immunohistochmisty technique and MIAS image analysis system. Results： The expression of CGRP in neurofibrils in the wound tissue（ 1,2,4,8,12,24 h） was higher than that in the control （P 〈 0.05 ）. However, in postmortem injury group, there were no significant changes in anti-CGRP immounreaetivities in neurofibrils compared to the control group（P 〉 0.05 ）. Further, the expression of CGRP could be detected increasingly in 1 h, and reached maximum in 4 h, returned in 8 h, then returned to normal there after. However, the expression of VEGF could be detected increasingly in 8 h after injury, and reached maximun in 72 h（P 〈0.01 ） ,then returned in 96 h. Conclusion： The expression of CGRP was erlier than VEGF in rat＇s skin after injury, and it was time dependent. It could used as a bio-marker to determine the time of injury in the early wound.