锰对小鼠肝肾的致毒作用

Toxic mechanism in the liver and kidney of mice induced by manganese

目的:探讨锰对肝脏和肾脏的毒性机理,防治锰中毒。方法:测定锰染毒小鼠的血清天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)和乳酸脱氢酶(LDH)活性、肝脏与肾脏脂质过氧化物值(LPO)和肝、肾组织中锰、锌、铁等浓度。结果:染锰小鼠血清AST,ALT,LDH活性、肝脏与肾脏LPO值和锰含量均明显高于对照组,差异有统计学意义,而肝、肾中铁、锌含量明显低于对照组(P<0.01),说明锰具有明显的肝、肾毒性。结论:脂质过氧化及锌、铁等代谢障碍可能是锰引起肝肾损害的重要机理。

Objective： To study the mechanism of Mn-induced toxicity in the liver and the kidney so to prevent or treat Mn poisoning. Methods： The activity of aspartate aminotransferase （ AST）, alanine aminotransferase（ALT） and lactate dehydrogenase （LDH） of the serum was tested, and the lipid peroxidation （LPO） of the liver and the kidney as well as the concentrations of manganese （ Mn）, zinc （Zn） and iron （Fe） in the liver and kidney of the mice exposed to Mn were studied. Results： The AST, ALT, LDH, LPO and concentrations of Mn in the liver and kidney of the mice in the Mn exposed groups were significantly higher than those of controls, but concentrations of Fe and Zn were significantly decreased. Mn has obvious toxicity to the liver and the kidney. Conclusion： Lipid peroxidation and metabolic dysfunction of Zn and Fe maybe the important mechanism of the liver injury and the kidney injury caused by Mn.