摘要
目的：探讨巨噬细胞炎症蛋白-1（MIP-1）在非小细胞肺癌组织中的表达与肿瘤浸润树突状细胞之间的关系。方法：经外科手术切除的60例非小细胞肺癌组织标本，分别采用免疫组织化学检测趋化因子受体CCR1，CCR7的表达，荧光原位杂交法检测MIP-1的表达。结果：（1）肺癌细胞胞质及胞膜表达MIP-1，同时检测到肺癌组织中有CCR1和CCR7阳性树突状细胞的浸润。（2）肺癌组织中CCR1，CCR7及MIP-1表达和肺癌临床分期、淋巴结转移有关，和病理类型、分化程度无关。有淋巴结转移组表达MIP-1的肿瘤细胞及CCR1阳性树突状细胞数量明显高于无淋巴结转移组（P〈0．05），Ⅲ～Ⅳ期患者表达MIP-1的肿瘤细胞及CCRI阳性树突状细胞数量明显高于Ⅰ～Ⅱ期（P〈0．05）。（3）肺癌组织中MIP-1阳性细胞表达率和CCR1阳性树突状细胞数量呈正相关，和CCR7阳性树突状细胞数量呈负相关（P〈0．05）。结论：非小细胞肺癌组织MIP-1表达和肺癌临床分期及淋巴结转移有关，与肿瘤浸润树突状细胞表面分子CCR1，CCR7的表达关系密切，推测MIP-1可能影响肿瘤浸润树突状细胞的发育和趋化功能并参与肺癌的免疫逃逸和疾病进展。
Objective： To explore the relationship of dendritic cell infiltration and MIP-1 expression in non-small cell lung cancer（NSCLC） and its clinical significance. Methods： Immunohistochemical staining was performed to detect expression of CCR1, CCR7, and fluorescent in situ hybridization performed to detect expression of MIP-1 in 60 lung cancer tissues. Results： （ 1 ） MIP-1 positive tumor cells and CCR1, CCR7 positive dendritic cells were detected in lung cancer tissues ; （2） Expression rate of CCR1 and MIP-1 with lymph node metastasis was higher than that without lymph node metastasis, and stage Ⅲ- Ⅳ were higher than those of stage Ⅰ - Ⅱ, all above differences were statistically significant （ P 〈 0.05 ） ; （4） The positive percentage of CCR1, CCR7 and MIP-1 had no relationship with pathological type and cell differentiation（P 〉 0.05 ）, but was correlated with clinical stage and lymph node metastases （P 〈 0.05 ） ; The expression levels of MIP-1 of lung cancer were positive correlation with the percentage of CCR1 positive dendritic cells（P 〈 0.05 ） and negative correlation with the percentage of CCR7 positive dendritic cells （P 〈 0.05 ）. Conclusion： The expression of MIP-1 is related to lymph node metastasis and clinical stage of lung cancer. MIP-1 may play an important role of tumor immune escape and progression of disease through interfering cell differentiation of TIDC.
出处
《江苏大学学报（医学版）》
CAS
2008年第5期440-443,I0002,共5页
Journal of Jiangsu University：Medicine Edition
基金
吴江市科技局立项项目(ws2003132)
