摘要
目的:同时测定复方珍珠咽喉宁制剂中的梓醇和甘草酸含量,以控制复方珍珠咽喉宁含片质量.方法:超声提取,采用ODS-C_(18)反相柱(4.6mm×250mm,5μm),分别用流动相水-乙腈(99.4:0.6)和乙腈-ψ(乙酸)0.1%的水溶液(33:67),检测波长(λ)分别为210 nm和254 nm对梓醇和甘草酸进行测定。结果:采用ψ(甲醇)50%为溶剂,超声30 min,梓醇的平均回收率98.33%,RSD 0.78%;甘草酸的平均回收率96.89%,RSD 0.45%.结论:该法简便、快速、专属性强,适用于咽喉宁含片的含量控制。
Objective: To establish a method for determination of glycyrrhizin and catalpol in Zhenzhu yanhouning buccal tablets by HPLC. Methods: Glycyrrhizin and catalpol were determined by RP-HPLC on C18-column at 210 nm and 254 nm, with 0.6% acetonitrile and acetonitrile: 0.1% acetic acid solution (33 : 67) as mobile phase respectively. Results: The linearity of this method was good. The average recovery for glycyrrhizin and catalpol were 96.89%(RSD 0.45%) and 98.33%(RSD 0.78%),respectively. Conclusion: The established method is simple, quick and specific, which is suitable for the quality control of the compound drug.
出处
《抗感染药学》
2008年第3期163-166,共4页
Anti-infection Pharmacy
基金
"十一五"国家科技支撑计划(编号:2006BAI09B00)
浙江省科技厅重点攻关课题(编号:2004C1201)