摘要
To investigate the security of semen biologically, 15 bull semen samples were collected (of which 5 exhibited clinical signs of Foot-and-mouth disease) and identified by RT-PCR and virus isolation. The results indicated that the semen of the infected bulls were contaminated by Foot-and-mouth disease virus (FMDV), but FMDV was not detected in semen samples from those bulls not showing clinical signs of Foot-and-mouth disease (FMD). This is the first report of the presence of FMDV in bull semen due to natural infection in China. The analysis of the partial sequence of the VP1 gene showed that the virus strain isolated from semen has 97.9% identity with the virus isolated from vesicular liquid of infected bulls showing typical signs of FMD and belonged to the same gene sub-group.
To investigate the security of semen biologically, 15 bull semen samples were collected （of which 5 exhibited clinical signs of Foot-and-mouth disease） and identified by RT-PCR and virus isolation. The results indicated that the semen of the infected bulls were contaminated by Foot-and-mouth disease virus （FMDV）, but FMDV was not detected in semen samples from those bulls not showing clinical signs of Foot-and-mouth disease （FMD）. This is the first report of the presence of FMDV in bull semen due to natural infection in China. The analysis of the partial sequence of the VP1 gene showed that the virus strain isolated from semen has 97.9% identity with the virus isolated from vesicular liquid of infected bulls showing typical signs of FMD and belonged to the same gene sub-group
基金
State Science and Technology Support Program (2006DAD06A03)
Hi-tech Research and Development Program of China 863 (2006AA10A204).
关键词
口足病
病毒
临床分析
分析方法
治疗方法
Bull
Foot-and-mouth disease （FMD）
Foot-and-mouth disease virus （FMDV）
RT-PCR
Semen
