宫颈癌中EphA2，nm23-H1蛋白的表达与淋巴结转移的相关性研究

Expression of EphA2 Protein and nm23-H1 Protein in Cervical Cancer Associated With Lymph Node Metastasis

目的检测EphA2蛋白和nm23-H1蛋白在宫颈癌中的表达,探讨其与淋巴结转移的关系。方法本研究标本取自2002年3月至2005年12月在川北医学院附属医院妇产科手术切除的56例宫颈癌石蜡标本(实验组),采用免疫组化S-P法检测EphA2,nm23-H1蛋白在56例宫颈癌(12例有淋巴结转移,44例无淋巴结转移)患者石蜡标本和在12例正常宫颈组织标本(对照组)中的表达,分析EphA2和nm23-H1蛋白的表达与淋巴结转移的关系。结果本研究结果显示,EphA2蛋白实验组的阳性表达率(85.7%,48/56)明显高于对照组(25.0%,3/12)。实验组中,12例有淋巴结转移患者的EphA2蛋白的阳性率为100%,44例不伴随淋巴结转移患者EphA2蛋白的阳性率为81.8%(36/44),两者比较,差异有显著意义(P<0.05)。实验组nm23-H1蛋白的阳性表达率(48.2%,27/56)低于对照组(91.7%,11/12)。实验组中,12例有淋巴结转移患者的nm23-H1蛋白阳性表达率为8.3%(1/12),44例不伴有淋巴结转移患者的为59.1%(26/44),两者比较,差异有显著意义(P<0.01)。结论EphA2和nm23-H1蛋白在宫颈癌中的表达与患者有无淋巴结转移密切相关。这提示,EphA2和nm23-H1蛋白可望为宫颈癌患者预后,提供参考依据。

Objective To investigate the expression of EphA2 protein and nm23-H1 protein in cervical cancer and their relationship with lymph node metastasis. Methods 56 patients with cervical cancer （from March 2002 to December 2005, in the Department of Gynecology and Obstetrics, the Affiliated Hospital of North Sichuan Medical University）were enrolled in this study, including 12 cases with lymph node metastasis and 44 cases without lymph node metastasis. The expression of EphA2 protein and nm23-H1 protein in 56 cases of cervical cancer and 12 eases of normal cervical tissue （the control group） were detected by immunohistochemistry S-P method. Results The positive expression rate of EphA2 protein in 56 cases of cervical cancer（85. 7%,48/56） was significantly higher than that of the control group（25. 0%,3/12）（P〈0. 01）, and the positive rate of EphA2 in 12 cases with lymph node metastasis （100%）was higher than that in cases without lymph node metastasis （81. 8%,36/44）（P〈 0. 05）. The positive expression rate of nm23-H1 protein in cervical cancer（48.2% ,27/56）was significantly lower than that in the control group（91. 7%,11/12）（P〈.0.01）, and the positive rate of nm23-H1 in 12 cases with lymph node metastasis （8. 3%, 1/12）was lower than that in those without lymph node metastasis（59.1%,26/44）（P〈0.01）. Conclusion The expression of EphA2 protein is positively related to lymph node metastasis and nm23-H1 protein is negatively related to metastasis in cervical cancer, which may serve as a reference for the prognosis in cervical cancer.