恩诺沙星与中华绒螯蟹血浆蛋白结合率的研究

Study on binding capacity of Enrofloxacin (ENR) with blood plasma protein of Chinese mitten-handed crab,Eriocheir sinensis

为探索养殖过程中恩诺沙星（Enrofloxacin，ENR）施用于中华绒螯蟹（Eriocheirsinensis）中的药效情况，文章通过静置平衡透析法分析研究了恩诺沙星与中华绒螯蟹血浆的蛋白结合率。试验测得的中华绒螯蟹血浆蛋白浓度为63．95±0．29mg·mL^-1，蛋白分子量主要集中在66．2～97．1kDa之间，恩诺沙星与中华绒螯蟹血浆蛋白的结合率在47．39％-82．58％之间。分析结果认为，恩诺沙星与中华绒螯蟹血浆50％以上的蛋白结合率，在中华绒螯蟹养殖生产过程中需要慎重考虑恩诺沙星的有效药物浓度及其与其它药物的竞争作用，以能充分发挥恩诺沙星的作用。

The binding capacity of the drug enrofloxacin with the plasma protein of Chinese mitten-handed crab, Eriocheir sinensis during feeding was investigated. The method of fixed equilibrium dialysis test was applied to the study on the binding of enrofloxaein with the crab blood plasma protein. Concentration of the plasma proteins in Chinese mitten-handed crab was 63.95 ± 0. 29 mg·mL^-1. Molecular weight of these proteins were mainly between 66. 2 - 97. 1 kDa. Protein binding level of enrofloxacin and crab plasma proteins was 47. 39% ～ 82. 58%, showing that more than 50% of the drug could be bound to the proteins. Thus, it could be concluded that enrofloxacin can bind with plasma proteins tightly. So, in case of using enrofloxaein alone or with other medicine, its effective concentra- tion and competition between drugs should be considered in Chinese mitten-handed crab culture.