HPLC法测定无花果叶中补骨脂素的含量

Determination the Content of Psoralen in Leaf of Ficus Caracal by HPLC

目的:目的:建立无花果叶中补骨脂素含量测定的HPLC法。方法:色谱柱为Diamonsil C_(18)柱(150mm×4.6mm,5μm);流动相为:甲醇:水(62:38),流速1.0ml·min^(-1),检测波长245nm,柱温35℃。结果:补骨脂素在0.16～1.60μg范围内与峰面积呈良好线性关系(r=0.9999),平均回收率为96.8%,RSD为0.91%。结论:本方法简单、灵敏、准确,可作为无花果叶中补骨脂素含量的测定方法。

Objective：To establish an HPLC method to determine the content of psoralen in leaf of ficus caracal. Methods： Diamonsil C18 column（150mm×4.6mm,5μm） was used; The mobile phase was methanol：water（62：38）mixture; The flow rate was 1.0ml·min^-1; The temperature of column was set at 35℃; The detecting wavelength was at 245nm.Results：A good linear relationship was obtained for psoralen within the range of 0.16～l.60μg （r=0.999 9）.The average recovery was 96.8 % with RSD 0.91%. Conclusion：The method is simple,rapid and accurate. It can be used to determine the content of psoralen in leaf of ficus caracal.