纤维蛋白原Bβ-455G/A多态性与颈动脉粥样硬化斑块的相关性研究

Relationship between the Ppolymorphism of β-fibrinogen Gene-455G/A and Carotid Artery Atherosclerotic Plaque

目的研究纤维蛋白原Bβ(fibrinogen Bβ,FGB)基因启动子区域-455G/A变异与颈动脉粥样硬化斑块的关系。方法缺血性中风病人160例,其中颈动脉粥样硬化斑块病人68例(斑块组),颈动脉未见粥样硬化斑块者92例(非斑块组),应用多聚酶链反应加HaeⅢ内切酶检测FGB启动子区域-455G/A的多态性,血浆纤维蛋白原水平测定采用凝血酶原时间法,颈动脉斑块采用超声检测。结果A-455等位基因在斑块组的分布频率为0.17较非斑块组0.07明显增高(χ2=7.67,P<0.01)。不同组别的基因型的分布不同,G/A、A/A基因型在斑块组更常见(χ2=6.59,P<0.05)。斑块组血浆纤维蛋白原水平(4.82±1.21)g/L显著高于非斑块组(4.27g/L±0.90g/L,P<0.01)。结论血浆中纤维蛋白原水平受FGB基因-455G/A多态性的影响,A-455等位基因是颈动脉粥样硬化斑块形成的危险因素。

Objective To study the association between the variation of β - figrinogen gene promoter - 455G/A and carotid artery atherosclerotic plaque. Methods One hundred and sixty patients with ischemic stroke were divided into plaque group （n=68）, non - plaque group （n= 92）. Polymerase chain reaction with restrictive enzyme HaeⅢ （PCR- R LP method） was used to analyze the poly- morphism of β-figrinogen gene promoter-455G/A. Plasma fibrinogen levels were measured with prothrombin time （PT） test. Ca- rotid artery atherosclerotie plaque was detected with ultrasound. Results A-455 allele frequencies in the plaque group （0.17） were significantly higher than that in the non- plaque group （0.07, χ^2 = 7.67,P〈0.01）. The gcnetypes were significant difference in different groups （χ^2=6.59, P〈0.05）. The mean fibrinogen levels in plaque group （4.82 ±1.21） g/L were significantly higher than that in non - plaque group（4. 27 g/L±0. 90 g/L, t=3.29 ,P〈0.01）. Conclusion Plasma fibrinogen level could be affected by the β- fibrinogen gene - 455G/A polymorphism. And A - 455 allele might be an independent risk factor for carotid artery atherosclerotic plaque.