期刊文献+

VEGF基因转染同种异体骨髓间充质干细胞对心肌梗死的治疗作用 被引量:4

下载PDF
导出
摘要 目的研究血管内皮生长因子(VEGF)基因转染同种异体骨髓间充质干细胞(MSCs)对猪急性心肌梗死(AMI)的治疗作用。方法体外分离、纯化、培养猪骨髓MSCs,以PCNA染色标记细胞;制备、抽提、纯化质粒pd-VEGF。用球囊堵闭及拉伤冠状动脉法建立AMI模型1周后,随机将其分为4组,每组4只,进行经冠状动脉途径移植MSCs和/或VEGF转染。Ⅰ组给予转染VEGF腺病毒的MSCs移植;Ⅱ组单纯MSCs移植;Ⅲ组VEGF基因质粒;Ⅳ组DMEM对照组。4周后行免疫组化和超声心动图检查。结果Ⅰ组及Ⅱ组在梗死区及缺血区均可见较大量的PCNA标记的移植细胞。Ⅷ因子染色阳性的新生血管密度:Ⅰ组>Ⅲ组>Ⅱ组>Ⅳ组。4周后LVEF值:Ⅰ组>Ⅱ组>Ⅲ组>Ⅳ组(P<0.05或P<0.01)。结论VEGF基因转染同种异体骨髓MSCs移植猪心肌梗死区及缺血区可以提高缺血心肌的心功能,这可能和骨髓MSCs促进血管和心肌细胞的再生有关。
出处 《中西医结合心脑血管病杂志》 2008年第10期1177-1178,共2页 Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease
  • 相关文献

参考文献9

  • 1吴铁军,汤服民.骨髓间质干细胞移植治疗心肌梗死研究进展[J].实用医药杂志,2005,22(9):836-838. 被引量:2
  • 2Tomita S, Mickle DA, Weisel RD , et al. Improved heart function with myogenesis and angiogenesis after autologous porcine bone marrow stromal cell transplantation[J].J Thorac Cardiovasc Surg, 2002,123(6) : 1132 - 1140.
  • 3Orlic D, Kaj stura L, Chimentis, et al. Bone marrow ceils regenerate infarcted myoeardium[J].Nature,2001,410(4) :701 - 705.
  • 4Saito T,Kuang JQ, Bittira B,et al. Xenotransplant cardiac chimera:Immune tolerance of adult stem cells[J]. Ann Thorae Surg, 2002,74(1) :19 - 24.
  • 5Tse WT,Pendleton JD,Beyer WM, et al. Suppression of alsuppression of allogeneic T - cell proliferation by human marrow stromal cells:Imptications in transplantation[J]. Transplantation,2003,75 (3):389-397.
  • 6Meisel R,Zibert A, Laryea M,et al. Human bone marrow stromal cells inhibit allogeneic T - cell responses by indoleamine 2,3 - dioxygenase-mediated tryptophan degradation[J]. Blood, 2004, 103 (12) :4619 - 4621.
  • 7刘江,徐全,陈艰.G-CSF干预性治疗对大鼠急性心肌梗死区毛细血管形成的影响[J].江西医药,2006,41(8):550-552. 被引量:5
  • 8Vincenti V, Cassano C, Rocchi M, et al. Assignment of the vascular endothelial growth factor gene to human chromosome 6p213[J]. Circulation, 1996,93 (8) : 1493 - 1495.
  • 9Bull DA, Baikey SH, Rentz J J, et al. Effect of Terplex/VEGF- 165 genetherapy on left ventricular function and structure following myocardial infarction. VEGF gene therapy for myocardial infarction[J]. J Control Release,2003,93(2) :175 - 181.

二级参考文献24

  • 1Anversa P, Kajstura J. Ventricular myocytes are not terminally differentiated in the adult mammalian heart. Circ Res, 1998,83:1
  • 2Prockop DJ. Marrow stromal cells as stem cells for nonhematopoietic tissues. Science, 1997, 276(4):71
  • 3Pittenger F, Mackay A, Beck S, et al. Multilineage potential of adult human mesenchymal stem cells. Science, 1999, 284:143
  • 4Wakitani S, Saito T, Caplan AI. Myogenic cells derived from rat bone marrow mesenchymal stem cells exposed to 5-azacytidine.Muscle Nerve, 1995, 18:1417
  • 5Makino S, Fukuda K, Miyoshi S, et al. Cardiomyocytes can be generated from marrow stromal cells in vitro. J Clin Invest, 1999,103:697
  • 6Hakuno D,Fukuda K, Makino S,et al. Bone marrow-derived regenerated cardiomyocytes (CMG Cells)express functional adrenergic and muscarinic receptors. Circulation, 2002,105(3):380
  • 7Tomita S, Li RK, Weisel RD, et al. Autologous transplantation of bone marrow cells improves damaged heart function. Circulation,1999, 100(19 Suppl Ⅱ):Ⅱ247
  • 8Orlic D,KajsturabJ,Chimenti S,et al. Bone marrow cells regenerate infracted myocardium, Nature, 2001, 410:701
  • 9Taylor DA,Atkins BZ, Hungspreugs P,et al. Regenerating functional myocardium:improved performance after skeletal myoblast transplantation. Nat Med, 1998, 4(8): 929
  • 10Toma C, Pittenger F,Cahill KS,et al. Human mesenchymal stem cells differentiate to a cardiomyocyte phenotype in the adult murine heart. Circulation, 2002, 105(1):93

共引文献5

同被引文献47

引证文献4

二级引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部