异体骨膜细胞移植治疗股骨头缺血性坏死的实验研究

Treatment of Avascular Nectosis of Femoral Heads by Periosteal Cell Transplantation:an Experimental Study

目的：探讨骨膜细胞移植对股骨头缺血性坏死的修复作用．方法：在无菌条件下切取新生犬胫骨骨膜。进行骨膜细胞培养．用8只成年古老犬建立股骨头缺血性坏死模型、2周后，将培养的骨膜细胞移植到4只成年犬的股骨头缺血性坏死模型内，另4只不做骨膜细胞移植、6周后取出坏死后的股骨头进行组织病理学检查。结果：移植骨膜细胞的股骨头内有大量新骨形成，新生骨质呈小梁状排列，小梁表面常有单层的成骨细胞覆盖，提示新骨形成活跃；而不移植细胞的股骨头内无新骨形成．结论：异体骨膜细胞移植能促进骨再生，对缺血性骨坏死具有修复作用．

Aim:To inveshgate the effect of periosteal cell transplantation on the repair of avascular necrosis of femoral head (ANFH). Methods:The tibial periosteum of new bom dog were excised and cultured in vitro. Eight adult dogs were used. The animal model of ANFH had been made by transfening the freed famoral head into the muscle under the ilium on one side in each dog.Two weeks after the establishment Of ANFH, the cultured periosteal cells were collected and transplanted into the necrotic femoral heads in 4 dogs, and the femoral heads without periosteal cell transpanation in the other 4 dogs served as the control. Six weeks aller cell transpanation, the femoral heads were taken out and histologic examination was made. Results:There were active bone formation in the femoral heads with cell transplanation. The new bone was arranged into bone trabeculae most of which were covered by a monolayer of osteoblasts. In the contyol grorp, there was no new bone formation. Concusion: Periosteal cell transplantation can improve the repair of ANFH.