帕金森病脑脊液蛋白质组学研究

Proteomic studies in CSF of Parkinson's disease.

目的寻找头痛和帕金森病（PD）患者用药前、后脑脊液差异蛋白质点，确定PD疾病特异性蛋白（DSPs）和药物治疗对蛋白质表达谱的影响。方法收集2004-2006年重庆医科大学附属第一医院神经内科收治的24例PD患者及12例对照组头痛患者脑脊液。用双向凝胶电泳（2D-PAGE）分离蛋白质，银染染色，分析图像寻找有意义的差异蛋白点；进行质谱（MS）鉴定。结果脑脊液蛋白质点主要集中在pH4～7，头痛组与帕金森用药前后脑脊液电泳图谱蛋白质点分布基本一致，符合临床脑脊液生化常规检查，甲状腺激素结合蛋白、载脂蛋白E在PD患者用药前后相对于对照组都消失，血清转铁蛋白在PD患者用药前后相对于对照组都明显上调。结论用药前后PD患者脑脊液蛋白质点与头痛组脑脊液蛋白质点存在差异，药物治疗对PD患者脑脊液蛋白质表达谱影响不明显。

Objective Analyze the two-dimensional gel electrophoresis （2D-PAGE）with cerebrospinal fluid （CSF）of Parkinson＇s disease patients （ treatment with or without drugs） and controls （ patients with headache）. Select significant differentially displayed protein spots and identify by MALDI-TOF-MS. Analyse the disease-specific proteins （DSPs）. Meth. otis Proteins extracted from CSF was focused immobilized pH gradient isoelectic focusing（1PG-IEF） as the first dimension, and then was electrophoresed in vertical flat sodium dodecyl sulphate polyacrylamide gel electrophoresis （SDSPAGE） as the second dimension. The protein spots in gels was visualized by silver staining gel followed by gel scanning and image analysis. Searching for differentially displayed protein spots then identifiying by MALDI-TOF-MS. Analyzing DSPs roles and effect. Results Satisfied 2D-PAGE patterns of protein in CSF with PD patients and controls was acquired in the experiment. Using Pdquest software analyze the 2D-PAGE and findout three DSPs. They are transthyretin, apolipoprotein E and serotransferrin. Conclusion There are some significant difference protein spots in CSF with Parkinson＇s Disease and controls. Durgs have little effective to protein patterns in CSF.