双液相系统中生物降解植物甾醇制备雄烯二酮工艺

Preparing Androstenones by Microbial Degradation of Phytosterol in Biphasic System

在双液相系统中,研究植物甾醇生物降解制备雄烯二酮(AD)的工艺,并考察底物投料量以及添加表面活性剂、羟化酶抑制剂对降解的影响。结果表明:葵花油是分枝杆菌降解甾醇生成AD的最佳有机溶剂,底物投料量以及羟化酶抑制剂对转化有一定的影响。通过正交试验,确定了葵花油/水系统最优转化条件:油相浓度为20%,底物投料量为0.4%,添加剂为0.005%氯化汞,此工艺条件下,AD转化率高达83.4%。

The technology of obtaining androstenones （AD） from phytosterol by microbial degradation in biphasic systems and the effects of substrate concentration, surfactants and 9α-hydroxylase inhibitor on hiotransformation were studied by experiment. The results show that sunflower oil-aqueous biphasic system is the most suitable for bioconversion, and the concentration of substrate and low-concentration of 9α-hydroxylase inhibitor can increase the rate of hiotransformation to a certain extent. Finally, the sunflower oil-aqueous biphasic system of fermentation is optimized by orthogonal experiment, and the optimal condition is as following： systems with the concentration of 20% sunflower oil, 0.4% substrate and 0. 005 % HgCl2. Under this condition the transformation rate of AD is 83.4 %.