摘要
The effect of different oxygen supply levels by controlling flask capacity on the performance of producing L-arginine using Corynebacterium crenatum SYPA5-5 was investigated in the batch fermentation process.The analysis of fermentation kinetic models and metabolic flux indicated that the hexose monophosphate (HMP) pathway was much more active to produce nicotinamide adenine dinucleotide phosphate (NADPH) for bacterial growth at a high oxygen supply level.L-arginine production was also enhanced at a high oxygen supply level because of high glucose consumption.On the other hand,there was more metabolic flux from α-KG to Glu,the precursor of Arg.
The effect of different oxygen supply levels by controlling flask capacity on the performance of producing L-arginine using Corynebacterium crenatum SYPA5-5 was investigated in the batch fermentation process. The analysis of fermentation kinetic models and metabolic flux indicated that the hexose monophosphate （HMP） pathway was much more active to produce nicotinamide adenine dinucleotide phosphate （NADPH） for bacterial growth at a high oxygen supply level. L-arginine production was also enhanced at a high oxygen supply level because of high glucose consumption. On the other hand, there was more metabolic flux from α-KG to Glu, the precursor of Arg.
出处
《化工学报》
EI
CAS
CSCD
北大核心
2008年第9期2295-2301,共7页
CIESC Journal
基金
国家高技术研究发展计划项目(2007AA02Z207
2006AA020301)
国家重点基础研究发展计划项目(2007CB707804)
教育部新世纪优秀人才支持计划项目(NCET-07-0380)~~
关键词
L-精氨酸
钝齿棒杆菌
发酵
供氧
L-arginine
Corynebacterium crenatum
fermentation
oxygen supply
