人鼻黏膜上皮细胞2种原代体外培养方案的比较研究

Comparative study of two primary culture methods of human nasal epithelial cells in vitro

目的:比较人鼻黏膜上皮细胞(HNE)2种常用的体外培养方法的效果,探索适宜的HNE体外培养方案。方法:比较组织块培养方案和分离细胞培养方案的HNE体外培养成功率和生长曲线,进行HNE的形态学观察和细胞来源鉴定,以初步了解其生物学特性。结果:分离细胞培养方案的体外培养成功率(87.50%)优于组织块培养方案(83.33%),但差异无统计学意义(P>0.05)。分离细胞培养方案的HNE生长曲线较高于组织块培养方案。2种体外培养方案的HNE经细胞形态学观察和细胞角蛋白染色均证实为上皮细胞来源。结论:分离细胞培养方案细胞混杂少,增殖迅速,HNE获取稳定、可靠,较适宜原代培养研究。

Objective：To compare two primary culture methods of human nasal epithelial （HNE） in vitro, and explore a suitable method to be used in further study. Method： Achievement ratio and growth curve of primarily cultural HNE by tissue piece culture were compared to those by isolated cell culture. Shape and appearance were observed and cellular sources were identified to get preliminary bionomies of HNE. Result：The isolated cell culture method （87.5%） was shown to be superior to tissue piece culture method （83.33%） by comparing achievement ratios, but no statistical significance was found （P〉0.05）. The growth curve of isolated cell culture was higher than that of tissue piece culture. All cultured cells were confirmed coming from epithelial cells by observing shape, appearance and dyeing eytokeratin. Conclusion：The isolated cell culture method is more suitable for primary cul ture due to its less promiscuity, faster proliferation, and more stable and reliable cell supply.