Apaf-1和Caspase 9在大肠癌中的表达及意义

Expression of Apaf-1 and Caspase 9 gene in colorectal cancer and its significance

目的:探讨Apaf-1和Caspase 9在大肠癌中的表达和意义.方法:选用武汉市第三医院消化内科结肠镜切除的大肠腺癌组织60例,实时定量PCR方法检测大肠癌患者标本中Apaf-1和Caspase 9mRNA的表达.结果:Apaf-1和Caspase 9基因在癌组织中表达明显低于正常组织,而在癌旁组织及正常组织中表达相近.癌组织与癌旁组织相比,Apaf-1与Caspase 9基因表达明显下调(0.561±0.049vs0.947±0.077,0.412±0.014vs0.922±0.062,均P<0.01).结论:Apaf-1和Caspase 9可能在大肠癌的发生和发展过程中起着重要作用.

AIM： To investigate the significance of Apaf-1 and Caspase 9 expression in the carcinoma of large intestine. METHODS： Real-time quantitative reverse transcription polymerase chain reaction （RQ-RT-PCR） technique was established for detecting Apaf-1 and Caspase 9 mRNA expression in tissues incised from 60 colorectal cancer patients examined by colonoscopy in the third hospital of Wuhan City. RESULTS： Apaf-1 and Caspase 9 mRNA expression were significantly lower in colorectal cancer tissues than those in the normal tissues （0.561 ± 0.049 vs 0.947 ± 0.077, P 〈 0.01; 0.412 ± 0.014 vs 0.922 ± 0.062, P 〈 0.01）. The expression levels of Apaf-1 and Caspase 9 mRNA had no marked differences between the normal tissues and cancer-adjacent tissues. CONCLUSION： Apaf-1 and Caspase 9 gene dysfunction maybe contribute to the development and progression of colorectal cancer.