幽门螺杆菌感染与胃癌组织环氧合酶2表皮生长因子受体和血管内皮生长因子的表达

CorrelaUon of Helicobacter pylori infection with the expression of COX-2 and EGFR and VEGF in human gastric carcinoma

目的探讨环氧合酶2（COX-2）、表皮生长因子受体（EGFR）和血管内皮生长因子（VEGF）在胃癌组织中的表达，三者间的相关性及其与幽门螺杆菌（Hp）感染的关系。方法采用免疫组化法检测61例胃癌组织及相应的20例癌旁组织中COX-2、EGFR和VEGF的表达；采用Westernblot方法检测10例胃癌组织及其相应癌旁组织中COX-2、EGFR和VEGF蛋白的表达；用快速尿素酶诊断法和^13C呼气试验检测47例胃癌患者Hp感染状况。结果免疫组化检测结果显示，COX-2、EGFR和VEGF在胃癌组织中的阳性表达率分别为59．02％、36．07％、60．66％，均明显高于癌旁组织（分别为25．00％、0和30．00％；均P〈0．05），三者在胃癌组织中的表达且呈正相关。COX-2和EGFR在Hp感染阳性胃癌组织中的阳性表达率分别为75．76％和45．45％，高于Hp感染阴性者（28．57％和14．29％；均P〈0．05）。Western blot检测结果表明，胃癌组织中COX-2、EGFR和VEGF的灰度值分别为51．29±23．42、35．89±12．50和48．55±10．33，均显著高于癌旁组织（均P〈0．05）。结论COX-2、EGFR和VEGF在胃癌组织中高表达，Hp感染可能上调胃癌组织中COX-2、EGFR的表达。

Objective To investigate the correlation of Helicobactor trylori （Hp） infection with the expression of COX-2, EGFR and VEGF in human gastric carcinoma. Methods The expression of COX-2, EGFR and VEGF was detected by immunohistocbemistry in samples of 61 gastric cancers and 20 canceradjacent tissues. Western blotting was performed in samples of 10 gastric cancers and corresponding canceradjacent tissues. Hp infection was detected in 47 patients by fast urea enzyme test and 13C breath test. Results The results of immunohistochemistry showed that the positive expressions of COX-2, EGFR and VEGF in gastric carcinoma were 59.02%, 36.07% and 60.66% ,respectively, significantly higher than that in the normal mucosa （25.00%, 0 and 30.00%, respectively）. There was a positive correlation between the expression of COX-2, EGFR and VEGF and gastric carcinoma. The expression of COX-2 and EGFR was 75.76% and 45.45% in the gastric carcinomas with Hp infection, significantly higher than that in those without （28.57% and 14.29% ）. The protein expression of COX-2, EGFR and VEGF detected by Western blot in gastric carcinomas was also significantly higher than that in normal mueosa. Conclusion COX-2, EGFR and VEGF are overexpressed in gastric carcinoma, and there is a positive correlation among them. Hp infection may upregulate the expression of COX-2 and EGFR in gastric cancer tissues.