SBR生物除磷系统中聚磷菌的特性研究

Investigation on Characteristics of Phosphate-accumulating Organisms in SBR

采用人工合成污水,在两个分别以厌氧/好氧(A/O)和厌氧/缺氧(A/A)运行的SBR反应器中进行生物除磷诱导与强化,并对聚磷菌(PAOs)的特性进行了研究。采用传统分离方法从这两个运行稳定且可高效除磷的SBR反应器中获得了37株纯种菌,经异染颗粒和聚β-羟基丁酸(PHB)颗粒染色及纯种培养后,确定其中7株为高效聚磷菌。通过生理生化特性试验,确定SYO-1、SYA-2、SYA-5和FCO-2为假单胞菌属(Pseudomonas),SYO-2为棒杆菌属(Coryne-bacterium),SYO-8为肠杆菌科(Enterobacter),FCO-12为葡萄球菌属(Staphylococcus)。其中SYO-1、SYO-2、SYO-8、FCO-2、FCO-12为传统好氧聚磷菌,SYA-2和SYA-5为反硝化聚磷菌(DNPAOs)。以A/O方式运行的SBR反应器中细菌多样性和聚磷菌种类都较以A/A方式运行的SBR的多。在以A/A方式运行的SBR系统中,反硝化聚磷菌主要为假单胞菌属;而在以A/O方式运行的SBR系统中,除假单胞菌属外,还存在棒杆菌属、肠杆菌科和葡萄球菌属。

To study the characteristics of phosphate-accumulating organisms （PAOs） in the activated sludge system, two sequencing batch reactors （SBRs） were fed with synthetic wastewater and operated under anaerobic-aerobic and anaerobic-anoxic conditions, and the biological phosphorus removal performance was investigated. Thirty-seven strains were isolated by the traditional method from the stable and effective SBRs. By investigating poly-P and polyhydroxybutyrate （PHB） granule stains as well as behaviors of phosphate release and uptake under anaerobic and aerobic conditions in the liquid culture medium, seven of the thirty-seven strains are confirmed to be PAOs. Strains SYO-1, SYA-2, SYA-5 and FCO-2 are identified as Pseudomonas sp. , SYO-2 as Corynebacterium sp. , SYO-8 as Enterobacter sp. , FCO-12 as Staphylococcus sp. from physiological and biochemical testing results. Furthermore, strains SYO-1, SYO-2, SYO-8, FCO-2 and FCO-12 are identified as traditional aerobic PAOs, and SYA-2, SYA-5 as denitrifying phosphate-accumulating organisms （DNPAOs）. Both the biodiversity of bacteria and PAOs in the A/O system are higher than those in the A/A system. DNPAOs in the A/A system are Pseudomonas, but in the A/O system, besides Pseudomonas, bacteria such as Corynebacterium, Enterobacter and Staphylococcus also exist.