法洛四联征右室流出道肥厚心肌细胞凋亡、Bax及Bcl-2蛋白表达情况

Apoptosis of tetralogy of Fallot right ventricular outflow myocardial hypertrophy and expression of Bax and Bc1-2

目的研究法洛四联征右室流出道肥厚心肌细胞凋亡、Bax及Bcl-2蛋白表达情况,并探讨其对手术时机的选择的意义。方法36例法洛四联征按年龄分成三个组。A组:≤2岁组;B组:>2岁,且≤10岁;C组:>10岁。用末端标记原位细胞凋亡法和免疫组化法检测其右室流出道肥厚心肌细胞凋亡指数,Bax及Bcl-2蛋白表达水平。结果心肌细胞凋亡指数,A组与B组和C组相比较(P<0.05),A组最小。随年龄增长,Bax表达明显增多(P<0.05),Bcl-2表达先增后减,但是Bcl-2/Bax比值随年龄增长而逐渐减少(P<0.05)。结论不同年龄阶段法洛四联征右室流出道肥厚心肌细胞凋亡指数、Bax、Bcl-2蛋白表达存在明显差异。2岁内进行根治手术,预后较好。

Objective To study the tetralogy of Fallot （TOF） right ventricular outflow myocardial hypertrophy apoptosis, Bax and Bcl - 2 protein expression, and the significance on operative timing. Methods Thirty - three Cases, aged, were divided into three groups. A ： ≤ 2 - year - old group, B ： 2 - year - old 〈 and ≤ 10 - year - old, C ： 〉 10 - year - old. With apoptosis in situ end labeling law and immunohistochemical method the fight outflow tract myocardial hypertrophy apoptosis index was detected. Results Bax and Bcl - 2 protein levels. Myocardial apoptosis index of group A （ P 〈 0.05 ） is the smallest compared with Group B and Group C. With age, Bax expression increased significantly （ P 〈 0.05 ）, Bcl - 2 expression first rose and then fell, but Bcl - 2/Bax ratio gradually decreased with age （ P 〈 0.05 ）. Conclusions There are great differences between TOF right ventricular outflow myocardial hypertrophy apoptosis index, Bax, Bcl - 2 protein expression. The better the prognosis can be achieved by radical surgery within 2 - year - old.