丙泊酚对胆道感染患者髓样细胞触发受体-1表达的影响

Effect of propofol on the expression of TREM-1 in patients with biliary infection

目的探讨丙泊酚对胆道感染患者外周血髓样细胞触发受体-1(TREM-1)的影响。方法 48例严重胆道感染患者,随机分为两组:丙泊酚处理组(P 组,n=28),术中用丙泊酚6 mg·kg^(-1)·h^(-1)及异氟醚(呼气末异氟醚浓度维持在1%)维持麻醉;对照组(N 组,n=20),术中用异氟醚维持麻醉(呼气末异氟醚浓度维持在2.5%)。上述两组术中均间断静脉注射阿曲库铵、芬太尼维持麻醉.且用量无差异。两组分别在手术前及手术后12、24、48、72 h 留取血液标本,用流式细胞术检测外周血 TREM-1蛋白的表达;用酶联免疫吸附实验法(ELISA)检测血肿瘤坏死因子α(TNF-α)表达水平。结果术前两组患者的 TREM-1及 TNF-α含量差异无统计学意义;P 组患者术后12、24、48、72 h 的 TREM-1及 TNF-α水平均明显低于 N 组(P<0.05)。结论严重胆道感染患者可致外周血TREM-1蛋白高水平表达。丙泊酚可降低 TREM-1蛋白表达水平.减轻炎症反应。

Objective To investigate the effects of propofol on the expression of triggering receptor expressed on myeloid cells （TREM-1）after severe biliary infection patients. Methods Peripheral blood of 48 patients with biliary infection were collected. Fourty-eight patients were randomly divided into two groups ： propofol treated group （ P, n = 28） and control group （ N, n = 20） （2.5 % isaflurane inhalation anesthesia during operation）. The peripheral blood were collected before operation and at 12, 24, 48, 72 h after operation. The TREM-1 protein was detected by flow cytometry. Meanwhile,the serum TNF-αwas determined by ELISA method. Results The expression of TREM-1 was down-regulated in group P,compared with that in group N （P〈0.05）. The level of TNF-α in group P was much lower than that in group N （P〈 0.05）. Conclusion Severe biliary infection may lead to remarkable up-regulation of TREM-1 expression. Propofol anesthesia reduces the expression of TREM-1 protein in the early phase of inflammation.