白毒鹅膏菌漆酶的诱导及其对直接黑G的脱色作用

Inducement of laccase production by amanita verna and its use for the decolorization of azo dye

白毒鹅膏菌是一种重要的产生胞外漆酶的菌株。探讨了漆酶在各种营养条件下的分泌情况以及对直接黑染料的降解情况。结果表明,以麸皮作为碳源,酵母膏作为氮源,在120r/min的转速下培养,漆酶的产量最高。另外染料在较低质量浓度(10mg/L)时,漆酶对染料的降解迅速而彻底,在pH=4.5、50℃条件下,偶氮染料直接黑G能被粗酶液(10U/L)有效地降解,在120r/min时降解率达到84.4%。

Amanita verna is an excellent producer of the industrially important enzyme laccase. The secretion and degradation of direct black dye under various nutritious conditions are discussed. When wheat bran and yeast extract are used as carbon source and nitrogen source respectively, and cultivated at 120 r/min, a higher yield level is obtained. In addition, when the concentration of the dye is lower（10 mg/L）, the effect of laccase on the degradation speed of dye is rapid and thorough. When the pH is 4.5 and temperature 50 ℃, azo dye direct black G can be degraded effectively by crude laccase（10 U/L）. And at 120 r/min, the degradation rate reaches 84.4%.