反相高效液相法测定非那西汀血浆浓度及药代动力学研究

HPLC assay for plasma phenacetin concentration:a marker of cytochrome P 450 IA 2 and evaluation for its pharmacokinetics

用反相高效液相法，对血浆非那西汀药物浓度的测定方法及其药代动力学进行了研究。实验采用Ｗａｔｅｒｓ公司ＨＰＬＣ系统，国产ＺＯＲＲＡＸＢＰＣＮ柱，以磷酸盐缓冲液／乙氰为流动相，内标法定量。本方法标准血浆线性关系良好（ｒ＝０９９９９），线性范围０７０～８９２８μｍｏｌ／Ｌ，最低检测量为１２５ｎｇ，平均方法回收率为９９９２％，浓度为８９２８μｍｏｌ／Ｌ、１１１６μｍｏｌ／Ｌ的提取回收率分别为９４８％、８３１％和８２４％；精密度考察批内变异系数（ＣＶ％）分别为０４９、３６５和４９０，批间变异系数（ＣＶ％）分别为３９３、３６４和５９３。

A sensitive and precise RP HPLC method for determination of plasma phenacetin, a marker of cytochrome P 450 IA 2,and for its pharmacokinetical study was developed. Waters HPLC system, home made ZORRAX BP CN column and phosphate buffer/acetonitrile as a mobile were used.Methodological evaluation showed that plasma phenacetin concentration between 0 70 and 89 28 μmol/L had a good linear confficient relation (r=0 9999). The lowest detectable concentration and amount were 0 70 μmol/L and 1 25 ng respectively.The average recovery of the method was 99 92%,and the extraction recovery for the concentration of 89 29 μmol/L,11 16 μmol/L and 2 79 μmol/L was 94 8%,83 1% and 82 4% respectively and the average coefficient variation of within run and between run was 3 07% and 4 50% respectively.