RP-HPLC法同时测定三七安宫胶囊中人参皂苷和三七皂苷的含量

RP-HPLC simultaneous determination of Panax notoginseng saponins and ginsenoside in Sanqiangong capsules

采用反相高效液相色谱法（RP-HPLC）同时测定了三七安宫胶囊中三七皂苷和人参皂苷的含量．实验中，用十八烷基硅烷键合硅胶作为填充剂，乙腈-0．2％磷酸梯度洗脱（0～30min，V乙腈：V磷酸=20：80；31～80min，V乙腈：V磷酸=30：70）作流动相，检测波长为203n／n，流速为1．0mL／min．结果表明：三七皂苷R1在0．42～2．10μg范围内线性关系良好（r=0．9996），平均回收率（n=5）为96．4％；人参皂苷Rg1在1．59～10．6μg范围内线性关系良好（r=0．9999），平均回收率（n=5）为97．7％；人参皂苷Rb1在1．497～9．98μg范围内线性关系良好（r=0．9997），平均回收率（n=5）为98．1％．

To establish simple and reliable RP-HPLC method for quantitative determination of Panax notoginseng saponins and ginsenoside in Sanqiangong capsules at the same time. C18 column was used. The mobile phase consisted of acetonitrile -0.2 % phosphoric acid（0- 30 min, V乙腈 ： V磷酸 = 20： 80; 31 -- 80 min, V乙腈 ： V磷酸 = 30： 70）. The flow rate was 1.0 mL/min. The UV detection wavelength was 203 nm. The calibration curve of notoginsenoside R1 was linear in the range of 0.42 to 2.10 μg（ r =0. 999 6）and the average recoveries（ n = 5）were 96.4 %. The calibration curve of ginsenoside Rg1 curve was linear in the range of 1.59 to 10.6 yg（ r =0. 999 9）and the average recoveries（ n = 5）were 97.7%. The calibration curve of ginsenoside Rbl was linear in the range of 1. 497 to 9.98 μg（ r =0. 999 7）and the average recoveries（ n = 5） were 98.1%.