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半定量RT-PCR法检测Wee1基因在水稻胚乳发育过程中的表达模式

Semi-Quantitative RT-PCR to Study the Expression of Wee1 During Development of Rice Endosperm
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摘要 通过半定量RT-PCR研究Wee1基因在水稻胚乳中的表达模式.结果发现Wee1基因的表达伴随水稻种子胚乳的整个发育过程.在所检测的几个时期中,授粉后第4 d(4 days after pollination,4 DAP)的胚乳中Wee1基因表达量最大;以后随着发育的进行,其表达量逐渐降低,直至15 DAP的胚乳中仍有微弱表达. The expression of Weel in rice endosperm was investigated by semi-quantitative RT-PCR. The resuits showed the expression of Wee1 reaches the maximum in the endosperm at 4 DAP（4 days after pollination,4 DAP）, as endosperm develops on, its expression fails down gradually ,but can still be detected of a very low level till 15 DAP.
作者 华树海 姜孝成 张春来 Adrain Slater
机构地区 湖南师范大学生命科学学院 英国德蒙特福大学健康与生命科学学院
出处 《湖南师范大学自然科学学报》 CAS 北大核心 2008年第3期83-87,共5页 Journal of Natural Science of Hunan Normal University
基金 湖南省自然科学基金资助项目(06JJ2032) 国家科技基础条件平台建设子项目资助(2005DKA21006)
关键词 Wee1 半定量RT—PCR 基因表达 胚乳发育 Wee1 Semi-quantitative RT-PCR gene expression endosperm development
分类号 R394 [医药卫生—医学遗传学]
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参考文献18

  • 1SORREL D A. A WEE1 homologue from Arabidopsis thaliana [J]. Planta, 2002, 215: 518-522.
  • 2DEVITTE W, MURRAY JAH. The plant cell cycle Annu Rev [J]. Plant Mol Biol,2003, 54: 235-264.
  • 3DE JAGER S M ,MURRAY JAH. Retinoblastoma proteins in plants [J]. Plant Mol Biol, 1999,41: 295-299.
  • 4GUTIERREZ C. G1 to S transition : more than a cell cycle engine switch[J]. Curt Opin Plant Biol,2002,5: 4-486.
  • 5NURSE P. Universalcontrolmechanlsm regulating onset of M-phase[ J ]. Nature , 1990,344:503-508.
  • 6GOULE K L. Phosphorylation at Thr167 is required for Schizosaccharomyces pombe p34cdc2 function [ J ]. EMBO J, 1991,10 : 3 297-3 309.
  • 7FEATHERSTONE C, RUSSEL P. Fission yeast p107 Weel mitotic inhibitor is a tyrosine/serine kinase [ J ]. Nature, 1991,349 : 808-811.
  • 8PARKER L L, ATHERTON-FESSLER S,PIWNICA-WORMS H. p107 Weel is a dual-speeifleity kinase that phosphorylates p34cdc2 on tyrosine 15[J]. Proe Natl Aead Sci U S A,1992, 89:2 917-2 921.
  • 9MEIJER M,MURRAY JAH. Cell cycle controls and the development of plant form[J]. Curt Opin Plant Biol ,2001, 4:44-49.
  • 10GBAFI G. Cell cycle regulation of DNA replication: the endoreduplication perspectlve[J]. Exp Cell Res ,1998, 244:372-378.

二级参考文献7

  • 1SALZMAN R A, FUJITA T, ZHU-SLAZMAN K, et al. An improved RNA isolation methed for plant tissues containing high levels of phenolic compounds or carbohydrates[J]. Plant Mol Biol Reptr, 1999, 17:11-17.
  • 2AINSWORTH C. Isolation of RNA from floral tissue of Rumex acetosa(Sorrel) [J]. Plant Mol Biol Reptr, 1993, 12: 198-201.
  • 3AINSWORTH C, CHARK J, BALSDON J. Expression, organization and structure of the genes encoding the waxy protein (granule-bound starch synthase) in wheat[J]. Plant Mol Biol,1993, 22: 67-82.
  • 4CHIRGWIN J M, PRZYBYLA A E, MCDONALD R J, et al. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease [J ]. Biochem, 1979, 18: 5294-5299.
  • 5KRIEG P A. A Laboratory Guide to RNA: Isolation, Analysis,and Synthesis[M]. New York: Wiley-Liss, Inc, 1996.21-41.
  • 6GAO Jian-wei, LIU Jian-zhong, LI Bin, et al. Isolation and purification of functional total RNA from Blue-grained wheat endosperm tissues containing high levels of starches and flavonoids [J]. Plant Molecular, 2001, 19:185 a-i.
  • 7黄培堂.分子克隆实验指南(第三版)[M].北京:科学出版社,2003.1655-1656.
湖南师范大学自然科学学报
2008年 第3期

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