锗-132对奶牛抗氧化系统功能的影响

Effects of Ge 132 on Antioxidant Function of Dairy Cattle

为了探讨锗１３２对奶牛抗氧化系统功能的影响，选用２５头奶牛，随机分组，进行３个月的实验，每组５头，共５组：Ⅰ组为对照组；Ⅱ组加锗１３２；Ⅲ组加锗１３２、硒；Ⅳ组加锗１３２、硒、锌；Ⅴ组加锗１３２、硒、锌、铜。定期采集血样，测定血清中ＧＳＨＰｘ、ＣｕＺｎＳＯＤ的活性、ＭＤＡ的含量以及日粮和全血中的Ｇｅ、Ｓｅ、Ｃｕ、Ｚｎ的含量。实验结果表明：加锗组血清ＧＳＨＰｘ、ＣｕＺｎＳＯＤ活性显著高于对照组（Ｐ＜００１），血清ＭＤＡ含量显著低于对照组（Ｐ＜００１）。全血锗含量与血清ＧＳＨＰｘ、ＣｕＺｎＳＯＤ和均呈显著正相关与血清ＭＤＡ含量呈显著负相关。证实锗１３２在奶牛体内具有抗氧化、清除自由基的作用；Ⅲ组血清ＧＳＨＰｘ活性高于Ⅱ组，且全血锗与硒含量呈明显正相关（γ＝０１９１７）。表明硒、锗在奶牛体内抗氧化作用方面有一定的协同作用。

Effects of β carboxyelgermanium sesquioxide (Ge 132) on the function of antioxidant system of dairy cattle and the relationship between antioxidant elements Ge and Se, Cu, Zn were studied with 25 dairy cattle fed on the same basal diets during three months experiment. The cattle were randomly divided into 5 groups, 5 for each. Group Ⅰ were used as control. Different elements were added into the diets for the following experimental groups, such as group Ⅱ Ge 132, group Ⅲ Ge 132 and Se, Group Ⅳ Ge 132, Se and Zn, Group Ⅴ Ge 132, Se, Zn and Cu. Blood samples were collected at regular intervals to examine the activities of serum GSH Px, CuZn SOD, the contents of serum MDA, blood Ge, Se, Zn, Cu, and the routine blood indexs as well. The experimental results showed as follows, the activities of serum GSH Px and CuZn SOD in group Ⅱ, Ⅲ, Ⅳ, Ⅴ were significantly higher than that in groupⅠ( P <0.01), and the contents of serum MDA in group Ⅱ, Ⅲ, Ⅳ, Ⅴ were significantly lower than that in groupⅠ( P <0.01), There were significantly positive correlation between the contents of blood Ge and the activities of serum GSH Px, CuZn SOD ( r =0.4997 and r =0.2502 respectively), but negative correlation between the contents of blood Ge and serum MDA levels ( r = 0.2996) in relative analysis, which indicates that Ge 132 performs the functions of antioxidation and scavenging free radicals. The activities of serum GSH Px in group Ⅲ were significantly higher than that in group Ⅱ, and there were significantly positive correlation between the contents of blood Ge and Se ( r =0.1071) which indicates that Se and Ge perform lhe synergic faction of antioxidation in dairy cattle.