海南沿海二种海藻抗细菌生物活性物质的提取及抑菌活性的研究

Research of extraction on anti-bacteria biological activity from two kinds of seaweed in Hainan coast

采用圆形纸片法以金黄色葡萄球菌(Staphylococcus aureaus)、枯草芽孢杆菌(Bacillus subtilis)、大肠埃希菌(Escherichia coli)、藤黄微球菌(Micrococcus luteus)、短小芽孢杆菌(Bacillus pumilis)5种细菌作为受试菌进行抗菌实验,对采自海南沿海的2种海藻的乙醚提取粗脂及乙醇提取物的脂溶性和水溶性物质进行抗细菌活性的研究。结果表明,江蓠藻乙醚提取的粗脂只对金黄色葡萄球菌有抑制,然而层析(柱层析法和薄层层析法对两种提取物进行层析分离)的所有组分对5种细菌具有较好的抗性。比之下马尾藻的三种粗提物都有抑制作用,其层析组分也能有很好的抑制作用,其中95%乙醇提取出的水溶性物质也有一定的抗菌作用。具体的活性物质还有待于进一步研究。

The two kinds of marine algae growing in Hainan＇ s coasts were studied on their antimicrobial capacity by Double-Disc method. Five bacterial Staphylococcus aureaus, Bacillus subtilis, Escherichia coli, Micrococcus luteus, Bacillus pumilis were used. The process was repeated by changing ether to 95% ethanol. The results showed that the coarse lipid of Acilaria verrueosa only inhibit Staphylococcus aureaus, but its Column chromatography separation can inhibit above five kinds of bacteria. In comparison, three kinds of coarse extracts of Sargassum polycystum can inhibit given bacteria while its Column chromatography separation also showed the inhibit ability on the bacteria. Further characterizations of these active substances need to be deeply studied.