铁蛋白在胎盘组织的定位及其在母-胎铁转运中的作用

Localization of ferritin in placental villous tissue and its role in the maternalfetal iron transport

目的：通过了解铁蛋白在胎盘组织中的定位及含量，探讨其在母胎铁转运中的作用。方法：采用免疫细胞化学分析的方法，对不同妊娠阶段的胎盘绒毛组织中铁蛋白的定位和分布进行了研究，并同时进行了胎盘微绒毛膜铁蛋白含量的放射免疫分析。结果：①胎盘合体细胞膜表面和基底膜上铁蛋白分布较多；②孕母患缺铁性贫血（ＩＤＡ）时胎盘组织中铁蛋白含量明显降低（Ｐ＜０．０１）。结论：铁蛋白可作为胎盘铁转运的重要载体，通过铁蛋白受体的介导，完成由母体经胎盘向胎儿的铁转运过程，而孕母ＩＤＡ时经胎盘的铁转运可能减少。实验还表明，随孕龄增加，胎盘微绒毛组织中铁蛋白含量增加，提示在妊娠过程中，经胎盘铁转运也是逐渐增加的。

Objective:To investigate the localization and quantity of ferritin in placental villous tissue and its implications.Methods:The localization of ferritin in placental villous tissue at different gestation period was assayed by immunocytochemistry.The quantity of ferritin in placental villous tissue was determined with radioimmunoassay.Results:①Ferritin was localized in all layers of the trophoblast,especially in the surface of the syncytiotrophoblast and basal membrane.②The quantity of ferritin in placental villous tissue was increased with gestation progress and decreased in pregnant women with IDA.Conclusions:①Ferritin may be the important carrier of transplacental iron transport,and its receptor plays a crucial role in the course.②The placental iron transport varied with gestation progress and iron nutritional status of the mother.