有限稀释法定量分析脐血中异基因特异的辅助性T细胞

Quantitative assessment of allospecific interleukin2secreting helper T lymphocyte in cord blood with limiting dilution assay

目的：探讨脐血移植是否会发生同成人骨髓移植一样强烈的移植物抗宿主病（ＧＶＨＤ）。方法：用有限稀释法检测了１７例脐血及１４例成人外周血中异基因特异的分泌白细胞介素２的辅助性Ｔ细胞（ＡＩＨＴＬ）。结果：脐血中ＡＩＨＴＬ频数与成人外周血相比无显著性差异（Ｐ＞０．０５）。结论：脐血的异基因反应性不比成人外周血差，故脐血移植尚需ＨＬＡ配型及移植后的免疫抑制治疗，以减少ＧＶＨＤ的发生。

Objective:In order to investigate whether cord blood transplantation could induce severe graft versus host disease(GVHD) similar to that in bone marrow transplantation.Methods:The frequency of allospecific IL2secreting helper T lymphocyte (AIHTL) in 17 cord blood and 14 adult peripherial blood samples were detected by limiting dilution assay.Results:No statistically significant difference between the cord blood and adult peripheral blood AIHTLs was revealed.Conclusion:Alloreactivity of cord blood was the same as that of adult blood.HLA typing and powerful immunosuppressive therapy should be adopted to reduce the incidence of GVHD in cord blood transplantation.