锰镁拮抗二氧化硅致成纤维细胞DNA损伤的实验研究

Experimental Study on Antagonism of Manganese and Magnesium against Fibroblast Cell DNA Damage Induced by Silicon Dioxide

目的观察氯化锰(MnCl2)、硫酸镁(MgSO4)及两者联合作用对二氧化硅(SiO2)致中国仓鼠肺成纤维细胞(CHL)DNA损伤的影响,寻找两者的最佳剂量组合。方法常规培养CHL细胞,按实验分组:生理盐水对照组(阴性对照组);SiO2组(100 mg/L);MnCl2组(1.0 mg/L);MgSO4组(2.0μmol/L);SiO2+MnCl2组(内含100 mg/L SiO2外,MnCl20.25、0.5和1.0 mg/L);SiO2+MgSO4组(内含100 mg/LSiO2外,MgSO40.5、1.0和2.0μmol/L);MnCl2、MgSO4联合作用组(每组内含SiO2100 mg/L外,采用两因素三水平正交设计,MnCl2+MgSO4共设9个剂量组)。培养2 h后收获细胞,通过彗星试验检测CHL细胞DNA损伤情况。结果与SiO2组比较,MnCl2、MgSO4可以明显减轻SiO2致DNA链断裂作用(P<0.05);1.0 mg/L MnCl2与1.0μmol/L MgSO4联合作用效果最好。结论MnCl2、MgSO4及两者联合应用在体外可有效减轻SiO2粉尘致CHL细胞的DNA损伤作用。

Objective To investigate either alone or joint antagonism of manganese chloride and magnesium sulfate against DNA damage induced by silicon dioxide on Chinese hamster lung cells. Methods Fibroblast cells were cultured with silica and manganese chloride （0.25, 0.5 and 1.0 mg/L） and magnesium sulfate （0.5, 1.0 and 2.0 /μmol /L） either alone or jointly. DNA damages were measured by comet assay. Results Both manganese chloride and magnesium sulfate alone, and manganese chloride jointly with manganese chloride showed antagonism against DNA damage induced by silicon dioxide on Chinese hamster lung cells. The most preferable dosage was found to be manganese chloride （1.0 mg/L） and magnesium sulfate （1.0 μmol/L）. Conclusions Manganese chloride and magnesium sulfate either alone or jointly could reduce DNA damage induced by silicon dioxide on Chinese hamster lung cells.