人牙髓细胞体外培养的方法学研究

Methodological study on primary culture of human dental pulp cells

作者观察了组织块法和酶消化法以及DMEM和RPMI1640二种常用培养液在人牙髓细胞体外培养中的效果。结果表明，组织块法培养出的牙髓细胞为单一的成纤维细胞样细胞，用DMEM时，其传代成功率明显高于用RPMI1604；胰蛋白酶消化法、胰蛋白酶和胶原酶联用可培养出成纤维细胞样细胞，少量的校形细胞和内皮样细胞，但细胞数量较少，生长缓慢，达不到传代要求。DMEM在培养板和组织培养瓶中均较适合牙髓细胞的生长，而RPMI1640效果不理想。提示组织块法和DMEM适合于人牙髓细胞的体外培养。

The purpose of this study was to compare the different effects of the explant and enzytnatic separation methods and twomedia- DMEM and RPMl 1640 on culturing human dental pulp cells. Pulp tissues came from normal first premolars ofadolescents. Results:A single population of fibroblast - like cells was obtained from the explant and the cells were easier tobe subcultured in DMEM (Five twelfth) than in RPMI 1640 (one twelfth). DMEM resulted in better cell growth both inplates and flasks than RPMI 1640. prominent fibroblast -like ceIls and minor colonies of spindle cells and endothelial - likecells were seen in enzpoatic separtion groupsl but these cells grew too slowly to reach confluence. Conclusion: The explanttechnique and DMEM were useful in culturing human dental pulp cells.