丹参肠内营养制剂对S-MODS模型大鼠肠黏膜上皮细胞凋亡影响

Study the Influence of Salvia Miltiorrhiza for Enteral Nutrition Praeparatum to Intestinal Mucosa Endothelial Cell Apoptosis in S-MODS Model Rats

目的:观察丹参肠内营养制剂对内毒素导致S-MODS模型大鼠肠黏膜上皮细胞凋亡影响,探索防治S-MODS新途径。方法:建立大鼠内毒素S-MODS模型,将WISTAR大鼠70只随机分为正常组(10只)、模型组(20只)、能全素组(20只)和丹参组(20只)。试验周期12d,试验第1d起分别用生理盐水、能全素以及丹参肠内营养制剂灌胃,试验第4d,正常组经鼠尾静脉注射生理盐水1mL/kg,其余3组经相同部位注射内毒素3mg/(mL·kg)(生理盐水溶解)。实验第11d停止灌胃。分别于静脉注射内毒素前1d、后1d和试验结束时测定白细胞计数,试验结束时石蜡包埋切片,用TUNREL方法检测肠黏膜上皮细胞凋亡情况。结果:通过临床表现以及白细胞计数、病理结果显示S-MODS造模成功,试验结束时与注射内毒素后1d相比,丹参组临床症状显著改善,试验结束时丹参组白细胞计数升高数目以及小肠黏膜上皮细胞凋亡指数明显低于模型组以及能全素组。结论:丹参肠内营养制剂能阻止肠道屏障破坏,保护肠黏膜,能减轻S-MODS模型大鼠全身炎症反应。

Objective ：To study the influence of salvia miltiorrhiza for enteral nutrition praeparatum to intestinal mucosa endothelial cell apoptosis in S-MODS model rats, and to explore a new treatment to prevent S-MODS. Methods： 70 Wistar rats were randomly divided into four groups： control group （n=lO）, model group （n=20）, nutrition fibre group （n=20） and salvia miltiorrhiza for enteral nutrition group （n=20）. The experiment period was 12 days. They were given Sodium Chloride, Sodium Chloride, nutrition fibre and salvia miltiorrhiza independently by intragastrie administration for EN every day for 11 days. On the fourth day, sodium chloride was administered intravenously in normal group and endotoxin 3 mg/kg was administered intravenously in other groups. Blood routine was measured at 24 hours before and after giving the endotoxin. At the end of experiment, blood routine was measured. We use Paraffin imbedding chip when experiment completed. TUNREL method was used to detect intestinal mueosa endothelial cell apoptosis. Results： The model succeed by clinical manifestation, blood routine and pathology. We observed that compared with other groups after injection endotoxin 24 hours, clinical symptom was improved, blood routine recuperated in salvia miltiorrhiza for enteral nutrition group at the end of experiment. Blood leucocyte count and apoptotic index in salvia mihiorrhiza for EN group were lower than model group and nutrition fibre group at the end of experiment （P 〈 0.05 ）. Conclusions： Salvia mihiorrhiza for enteral nutrition could prevent gut function damage, protect intestinal mucosa and significantly decrease apoptotic. Salvia miltiorrhiza for enteral nutrition also could also relive the inflammatory response.