低剂量X射线全身照射诱导小鼠脾细胞蛋白分子变化

Alterations of proteins in murine splenic cells after low dose radiation

为了深入了解辐射对细胞蛋白质表达的影响，采用双向电泳技术检测低剂量Ｘ射线全身照射诱导小鼠脾细胞核及胞浆蛋白分子变化。结果表明，７５ｍＧｙＸ射线照射后４ｈ细胞核出现４个新蛋白点；２个增强蛋白点。照射后细胞浆出现６个新蛋白点；１个增强蛋白点和３个减弱蛋白点。正常细胞浆的３个蛋白点在照射后消失。正常细胞浆中的２个蛋白点在照射后分别减弱和消失，而在细胞核中增强和出现，提示低剂量辐射促进细胞核及细胞浆大分子物质的交换。

Alterations of proteins in the nuclear and cytoplasmic fractions of splenocytes were investigated in mice 4 hours after whole body irradiation with 75 mGy Xrays by 2Delectrophoresis.It was found that alterations of 6 proteins ranging in size from 12 to 43 kD with their pI distribution from 5.6 to 6.6 were displayed in the nuclear fraction of splenocytes in irradiated mice,of which there were 4 novel proteins and 2 upregulated proteins and at the same time alterations of 10 proteins ranging in size from 10 to 69 kD with their pI distribution from 5.3 to 8.2 were displayed in the cytoplasmic fraction of splenocytes in irradiated mice,of which there were 6 novel proteins,1 upregulated proteins and 3 downregulated proteins,and 3 proteins ranging in size from 24 to 32 kD with their pI distribution from 5.2 to 6.5 disappeared after radiation.It was shown that a 32 000/5.2(MW/pI) protein molecule in the shamirradiated cytosol of splenocytes appeared in the nuclei after radiation and a 43 000/6.3 protein molecule in the shamirradiated cytosol decreased and appeared in the nuclei after radiation.The results suggest that exchanges of protein molecules occurred between nuclear and cytoplasmic compartments after radiation.