秘灵王对Swiss鼠与裸鼠免疫功能的影响

Effect of Milingwang on immunofunctions of Swiss mice and naked mice

采用秘灵王（ＭＬＷ）灌喂正常Ｓｗｉｓｓ鼠与先天性免疫缺陷裸鼠，观察ＭＬＷ对两品系小鼠免疫功能的影响，探讨ＭＬＷ的免疫调节效应。结果表明，ＭＬＷ可显著促进Ｓｗｉｓｓ鼠Ｔ、Ｂ细胞对丝裂原的反应性；促进胸腺细胞与骨髓细胞的自发增殖；促进脾细胞产生ＩＬ－１、ＩＬ－６的能力（Ｐ＜０．０１）。ＭＬＷ对裸鼠仅可促进Ｂ细胞对ＧＰＳ的反应性（Ｐ＜０．０５）。裸鼠与Ｓｗｉｓｓ鼠比较，裸鼠Ｂ细胞对ＬＰＳ、ＧＰＳ的反应性、骨髓细胞自发增殖、血清ＣＩＣ水平等指标均高于Ｓｗｉｓｓ鼠（０．０１＜Ｐ＜０．０５），而胸腺细胞自发增殖、血清溶菌酶含量、脾ＭΦ精氨酸酶水平均低于Ｓｗｉｓｓ鼠（Ｐ＜０．０５）。提示ＭＬＷ对正常鼠及免疫缺陷鼠有不同的免疫调节效应。

The immunofunctions of Swiss mice and nude mice administered with effective dose of Milingwang (MLW) agent in stomach were observed.The results showed that MLW could evidently promote the reactivity of T lymphocytes and B lymphocytes of Swiss mice to mitogens,sponteneous proliferation of thymus and bone marrow cells,and ability of producting interleukin-1 and interleukin-6 of splenocytes (P<001).MLW could only increase the reactivity of B lymphocytes of nude mice to glycyrrhiza uralensis fisch polysaccharide (GPS) (P<005).Compared to thoes of Swiss mice,nude mice′s parameters of reativity of B lymphocytes to lipopolysaccharide (LPS) and GPS,sponteneous proliferation of bone marrow cells and sera circle immune complex (CIC) level were higher (P<005).However, the parameters of sponteneous proliferation of thymocytes,sera lysozyme level,level of arginase in macrophages of spleen were lower (P<005).It may be suggested that MLW has different immune regulation effect on normal Swiss mice and immunodeficiency mice.