摘要
采用95%乙醇抽提、丙酮沉淀粗提新鲜牛心中的心磷脂;应用硅胶G高压梯度洗脱液对其进行纯化;应用硅胶G薄层层析进行了鉴定;应用修改的Fiske-Subbarow方法对纯化的心磷脂进行定量。结果显示硅胶G纯化的心磷脂纯度高,经薄层层析呈单一黄色斑点,定磷法定量产率为每100g新鲜牛心可获心磷脂8.23mg。结果提示:通过硅胶G层析法可获得高纯度、高产率的心磷脂。
In this experiment,crude phosphatides were acquired from 95% ethanol extracts from fresh bovine heart.The cardiolipin was separated from other phosphatides by column chromatography on silica gel G and identified using the method of TLC.The contents of cardiolipin were determined by the colorimetric determination of phosphorus.The results show that the method provides for a rapid,improved and convenient means for obtaining chromatographically cardiotipin.The yield of cardiolipin is 8.23 mg per 100 g fresh bovine heart tissue.
出处
《白求恩医科大学学报》
CSCD
1997年第6期681-683,共3页
Journal of Norman Bethune University of Medical Science
基金
国家自然科学基金