丙型肝炎病毒血清抗体分型研究

Study on hepatitis C virus serotyping

为探讨丙型肝炎病毒(HCV)血清抗体分型的可能性及其意义,采用酶联免疫技术(EIA)对140例丙型肝炎患者血清抗-HCV 型特异性抗体进行检测,并比较其与基因分型的检测结果。结果显示63.6%(89)的患者检测出 HCV 血清型特异性抗体,其中血清1型占87.6%(78/89),血清2型占6.7%(6/89),血清1+2型占5.6%(5/89)。对其中71例血清 HCV RNA 阳性者的血清型结果与基因型结果相比较,二者符合率达97.2%。研究表明 HCV 血清抗体分型简便易行,尤其可用于对部分抗-HCV 阳性而 HCV RNA 阴性患者进行分型。但其敏感性尚有待于提高。

To study the possibility and significance of HCV serotyping,EIA was used to detect type -specific antibodies of HCV in sera from 140 patients with viral hepatitis C,and the results were com- pared with that of HCV genotyping by PCR with core region type-specific primers.The results showed that HCV type-specific antibody was detected in 63.6% (89) patients,of whom 87.6% (78) were serotype 1,6.7% (6) were serotype 2 and 5.7% (5) were mixed serotype 1 and 2.Comparative evalua- tion of HCV serotyping and genotyping with sera from 71 HCV RNA-positive patients of these 89 suc- cessfully serotyped patients showed a remarkable consistence (97.2%)between HCV serotyping and genotyping.Our results suggested that serologic typing is a simple and practical assay to type HCV.Par- ticularly,it may be used to type HCV in some HCV hepatitis patients who are negative for serum HCV RNA.But the sensitivity of this method remains to be improved.