摘要
为了更全面地了解戊型肝炎病毒(HEV)的感染过程,从HEV开放读框1(ORF1)区设计两对寡核苷酸引物,建立逆转录-套式聚合酶链反应(RT-nPCR),对62例戊型肝炎患者发病后531份血清进行HEVRNA检测。结果显示,阳性患者占71%。全程随访32例患者288份系列血清,发现阳性24例(75%),其阳性率随病程延长而下降,平均持续时间为发病后20.6天。观察44例血清HEVRNA阳性患者,有36例(81.8%)在血清转氨酶和总胆红素水平开始回落时仍呈阳性;39例(88.6%)同时检测到抗-HEV。HEV血症的阳性率及阳性持续时间与血清转氨酶。
Hepatitis E virus RNA (HEV RNA) was detected in 531 serial serum specimens collected from 62 hospitalized patients with hepatitis E by reverse transcriptionnested polymerase chain reaction (RTnPCR) setting two pairs of primer from open reading frame 1 (ORF1) to understand the process of HEV infection more thoroughly. Results showed that 71 percent of the patients were positive for HEV RNA. Thirtytwo of them with 288 serial serum specimens were followed up for the whole process, and 24 cases (75%) were positive for HEV RNA, positive rates declined with the course of the disease and serum HEV RNA lasted for 20.6 days in average. Serum HEV RNA was kept positive in 36 of the 44 patients (81.8%) at the time their serum alanine aminotransferase (ALT) and serum total bilirubin began to lower, and antiHEV was detected in 39 of them (88.6%). Proportion of HEV viremia and length of it had no direct relationship with serum ALT and levels of total serum bilirubin and antiHEV.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
1997年第6期352-354,共3页
Chinese Journal of Preventive Medicine
基金
浙江省自然科学基金
浙江省卫生厅资助课题