Matriptase和HAI-1在膀胱移行细胞癌中的表达及其意义

Expression and value of Matriptase and HAI-1 in bladder transitional cell carcinoma

目的检测Matfiptase及HAI-1在膀胱移行细胞癌（BTCC）中的表达，探讨与BTCC临床病理参数间的关系及其临床意义。方法在同一组织标本两个石蜡切片中应用免疫组化技术检测Matriptase及HAI-1在BTCC及正常膀胱组织中的表达，并结合临床病理资料分析。结果Matfiptase及HAI-1在BTCC及正常膀胱组织中均有表达；两者在BTCC中的表达阳性率均明显低于正常膀胱组织，组间差异有统计学意义（P〈0．05）；HAI-1表达与肿瘤病理分级、临床分期呈负相关（相关系数r分别为-0．634，P〈0．001；-0．521，P〈0．001）。Matnptase表达随病理分级升高而降低，中、低分化组明显低于高分化组，差异有统计学意义（P〈0．05）；在不同临床分期组间差异无统计学意义（P〉0．05）。Matriptase和HAI-1在正常膀胱组织中表达相关系数r〈0．772（P〈0．001）；在BTCC中r〈0．546（P〈0．001）。结论Matfiptase和HAI-1在BTCC中表达降低，其异常表达在BTCC的发生发展过程中可能具有重要作用，HAI—i可作为判断BTCC恶性程度及预后的新型生物学标记，Matnptase在BTCC中的表达与促分化有关，可能是一种分化程度的指标。Matfiptase和HAI-1在BTCC中相关性降低，两者比例失衡可能在BTCC发生发展过程中具有重要作用。

Objective To investigate the expression and clinical significance of Matriptase and HAI-1 in bladder transitional cell carcinoma（ BTCC）, and to explore their relationship with the clinicopathological parameters of BTCC. Methods Expressions of Matriptase and HAI-1 protein in specimens of BTCC and normal bladder tissues were detected by SP method of immunohistochemistry. The results were analyzed in relation to the clinicopathological data. Results The expression of Matriptase and HAI-1 were observed in both normal and tumor tissues of bladder; The protein levels of Matriptase and HAI-1 were significantly higher in normal bladder tissues as compared with BTCC tissues （P〈0.05） ; a progressive decrease in the protein levels of HAI- 1 was observed with increasing of tumor grades （r=-0.634, P〈0.001） and clinical stages （r=-0.521, P〈0.001） ; the protein level of Matriptase was decreased with tumor grade increasing, and was significantly higher in high-grade tissues as compared with moderate and low-grade tissues （P〈0.05） ; Matriptase protein levels showed no significantly difference between different tumor clinical stages （P〉0.05）. The correlation coefficient of Matriptase and HAI-1 in normal tissues was 0. 772 （P〈0.001） and 0. 546 in tumor tissues of bladder（P〈0.001）. Conclusion The abnormal and decreased expressions of Matriptase and HAI-1 may be important events during the genesis and progression of BTCC in humans; HAI-1 may be used as a new parameter for assessing the malignancy and prognosis of BTCC; Matriptase protein in BTCC is related with differentiation promotion,and may be an indicator of differentiation. The correlation between Matriptase and HAI-1 decreases in BTCC. The imbalance between HAI-1 and Matriptase may be contributed to the progression of BTCC.