疑似盱眙水牛病自然病例病理形态与免疫机能的初步研究

PRELIMINARY STUDY OF PATHOMORPHOLOGY AND IMMUNE FUNCTION ON SPONTANEOUS CASES OF XUYU——LIKE BUFFALO DISEASE

对湖北8例疑似盱眙水牛病的病理形态与免疫机能变化的初步探究。结果表明,病牛外周血红细胞总数、血红蛋白含量、白细胞总数、中性白细胞及淋巴细胞总数均极显著减少(p<0.01),并伴有中性粒细胞核左移现象;淋巴细胞总数显著的减少,ANAE(十)淋巴细胞百分率则极显著提高(p<0.01),绝对值稍减少(0.1<p<0.5);T淋巴细胞对植物血凝素体外刺激反应的活性及血清总蛋白、白蛋白、γ球蛋白含量均稍低于正常,但差异不显著(0.05<p<0.1)。尸体剖检,可见肝、脾和所有淋巴结不同程度的变性,肿胀、多发性灶状坏死;组织学病变特征为中枢、外周免疫器官淋巴组织不同程度的变性、坏死;淋巴细胞,肝细胞的超微结构均表现为细胞器严重的退行性变化,在淋巴细胞的胞浆内,见断面呈六边形、大小69-83mμ,无明显囊膜的病毒粒子。

Eight spontaneous cases of Xuyu-like buffalo disease from Hubei Drovince werestudied on their pathomorphology and lmmune functions. The results indicated that thecirculating erythrocyte, hemoglobin determintion, leukocyte, neutrophil and lymphocytecounts wereall decreased significantly (p<0.01), and with marked left shift of neutrophil;the rate of ANAE positive lymphoyte was significantly increased (p<0.01); PHA stimulateder-ipheral blood T lymphoblastogenesis activities, total serum protein, albumin and, γ-glo-bulin value were only slightly decreased (p>0.05). Necropsy revealed different degreesof degeneration, enlargement, and multi-focal necrosis in liver, spleen and all the exa-mined lymphnodes. The characteristic histopatholgical changes were different degreel ofdegeneration and necrosis of lymphoreticular cells in all the examined tissues and orgars.The ultra-microscopical changes of lymphocyte and liver cell were all severe disintegra-tion of all organelles. In the cytoplasma oflymphocyte, virions were found with theshape of hexagonal sides, 69-83 nm in size and non-envelope.